Product Name	phospho-DDIT3 (Ser30)
Chinese Name	磷酸化GADD153抗体
Alias	DDIT3 (phospho S30); DDIT3 (phospho Ser30); DDIT3 (phospho-Ser30); p-DDIT3 (phospho S30); ; GADD153; CHOP; Growth arrest and DNA damage-inducible 153; C/EBP homologous protein; C/EBP Homology Protein; CEBPZ; CHOP10; DDIT 3; DNA Damage Inducible Transcript 3; GADD 153; Growth Arrest and DNA Damage Inducible Protein 153; Growth arrest and DNA damage inducible protein GADD153; MGC4154; DDIT3_HUMAN.
literatures	Specific References  (7)     |     SL5177R has been referenced in 7 publications. [IF=6.706] Yujie Zhong. et al. Diosgenin Ameliorated Type II Diabetes-Associated Nonalcoholic Fatty Liver Disease through Inhibiting De Novo Lipogenesis and Improving Fatty Acid Oxidation and Mitochondrial Function in Rats. NUTRIENTS. 2022 Jan;14(23):4994  WB ;  Rat.   PubMed:36501024 [IF=6.706] Yujie Zhong. et al. Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy. NUTRIENTS. 2023 Jan;15(9):2164  WB ;  Rat,Human.   PubMed:10.3390/nu15092164 [IF=6.284] Yujie Zhong. et al. Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca 2+ homeostasis. 2021 Mar 02  WB ;  Rat.   PubMed:33651226 [IF=6.023] Yujie Zhong. et al. Jujuboside A ameliorates high fat diet and streptozotocin induced diabetic nephropathy via suppressing oxidative stress, apoptosis, and enhancing autophagy. Food Chem Toxicol. 2022 Jan;159:112697  WB ;  Rat.   PubMed:34826549 [IF=5.396] Yujie Zhong. et al. Dioscin relieves diabetic nephropathy via suppressing oxidative stress, apoptosis, and improving mitochondrial quality and quantity control. Food Funct. 2022 Feb;:  WB ;  Rat.   PubMed:35262539 [IF=2.784] Yin M et al. Protective effects of CXCR3/HO‑1 gene‑modified BMMSCs on damaged intestinal epithelial cells: Role of the p38‑MAPK signaling pathway.Int J Mol Med. 2019 May;43(5):2086-2102.  IHC-P&ICC ;  Rat&Mouse.   PubMed:30864680 [IF=2.557] Ali Tugrul Akin. et al. Chloroquine attenuates chronic hypoxia-induced testicular damage via suppressing endoplasmic reticulum stress and apoptosis in experimental rat model. CLIN EXP PHARMACOL P. 2022 May 17  IHC ;  Rat.   PubMed:35579513
Product Type	Phosphorylated anti
Research Area	immunology  Signal transduction  Apoptosis  Cyclin  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Pig, Cow, Rabbit, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	19kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human GADD153 around the phosphorylation site of Ser30: VL(p-S)SD
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. Function: Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response. Subunit: Heterodimer. Interacts with TCF7L2/TCF4, EP300/P300, HDAC1, HDAC5 and HDAC6. Interacts with TRIB3 which blocks its association with EP300/P300. Interacts with FOXO3, CEBPB and ATF4. Subcellular Location: Cytoplasm. Nucleus. Note=Present in the cytoplasm under non-stressed conditions and ER stress leads to its nuclear accumulation. Tissue Specificity: Ubiquitinated, leading to its degradation by the proteasome. Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity Post-translational modifications: Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS. Similarity: Belongs to the bZIP family. Contains 1 bZIP (basic-leucine zipper) domain. SWISS: P35638 Gene ID: 1649 Database links: Entrez Gene: 1649 Human Entrez Gene: 13198 Mouse Entrez Gene: 29467 Rat Omim: 126337 Human SwissProt: P35638 Human SwissProt: P35639 Mouse SwissProt: Q62857 Rat Unigene: 505777 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-DDIT3 (Ser30)) Polyclonal Antibody, Unconjugated (SL5177R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Blank control (blue line): Hela(fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice) Primary Antibody (green line): Rabbit Anti-spho-DDIT3 (Ser30) antibody (SL5177R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

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