Product Name	phospho-APBB1 (Ser347)
Chinese Name	磷酸化铁蛋白Fe65抗体
Alias	Adaptor protein FE65a2; Amyloid beta (A4) precursor protein binding family B member 1; Amyloid beta A4 precursor protein binding family B; Amyloid beta A4 precursor protein binding family B member 1; Amyloid beta precursor protein binding family B member 1; APBB 1; APBB1; FE 65; Fe65 protein; MGC 9072; MGC9072; Protein Fe65; RIR; Stat like protein; Fe-65 Protein; APBB1_HUMAN.
Product Type	Phosphorylated anti
Research Area	Tumour  immunology  Neurobiology  Signal transduction  Apoptosis  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	77kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human APBB1 around the phosphorylation site of Ser347: SL(p-S)PE
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. Function: Transcription coregulator that can have both coactivatorand corepressor functions. Adapter protein that forms atranscriptionally active complex with the gamma-secretase-derivedamyloid precursor protein (APP) intracellular domain. Plays acentral role in the response to DNA damage by translocating to thenucleus and inducing apoptosis. May act by specifically recognizingand binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph)at double-strand breaks (DSBs), recruiting other pro-apoptosisfactors such as MAPK8/JNK1. Required for histone H4 acetylation atdouble-strand breaks (DSBs). Its ability to specifically bindmodified histones and chromatin modifying enzymes such asKAT5/TIP60, probably explains its trancription activation activity.Function in association with TSHZ3, SET and HDAC factors as atranscriptional repressor, that inhibits the expression of CASP4.Associates with chromatin in a region surrounding the CASP4transcriptional start site(s). Subunit: Component of a complex, at least composed of APBB1,RASD1/DEXRAS1 and APP. Interacts (via PID domain 2) with APP (withthe intracellular domain of the beta-amyloid precursor protein).Interacts (via PID domain 2) with RASD1/DEXRAS1; impairs thetrancription activation activity. Interacts (via PID domain 1) withKAT5/TIP60. Interacts (via the WW domain) with the proline-richregion of APBB1IP. Interacts with TSHZ1 and TSHZ2 (By similarity).Interacts (via the WW domain) with histone H2AX (whenphosphorylated on 'Tyr-142') and the proline-rich region of ENAH.Interacts with MAPK8. Interacts (via PID domain 1) with TSHZ3 (viahomeobox domain). Interacts with SET. Found in a trimeric complexwith HDAC1 and TSHZ3; the interaction between HDAC1 and APBB1 ismediated by TSHZ3. Interacts (via WWW domain) with NEK6. Interacts(via WWW domain) with ABL1. Subcellular Location: Cell membrane. Cytoplasm. Nucleus. Cellprojection, growth cone (By similarity). Nucleus speckle.Note=Colocalizes with TSHZ3 in axonal growth cone (By similarity).In normal conditions, it mainly localizes to the cytoplasm, while asmall fraction is tethered to the cell membrane via its interactionwith APP. Following exposure to DNA damaging agents, it is releasedfrom cell membrane and translocates to the nucleus. Nucleartranslocation is under the regulation of APP. Colocalizes withTSHZ3 in the nucleus. Co-localizes with NEK6 at the nuclearspeckles. Phosphorylation at Ser-610 by SGK1 promotes itslocalization to the nucleus Tissue Specificity: Highly expressed in brain; strongly reduced inpost-mortem elderly subjects with Alzheimer disease. Post-translational modifications: Phosphorylation at Ser-610 by SGK1 promotes its localizationto the nucleus (By similarity). Phosphorylated following nucleartranslocation. Phosphorylation at Tyr-547 by ABL1 enhancestranscriptional activation activity and reduces the affinity forRASD1/DEXRAS1. Similarity: Contains 2 PID domains. Contains 1 WW domain. SWISS: O00213 Gene ID: 322 Database links: Entrez Gene: 322 Human Entrez Gene: 11785 Mouse Entrez Gene: 29722 Rat Omim: 602709 Human SwissProt: O00213 Human SwissProt: Q9QXJ1 Mouse SwissProt: P46933 Rat Unigene: 372840 Human Unigene: 38469 Mouse Unigene: 19953 Rat Fe65 蛋白(又称作Amyloid beta A4 precursor protein-binding family B member 1)可能与细胞内的APP结构域结合调节内源性的APP。
Product Picture	Sample: U251(Human) Cell Lysate at 30 ug Primary: Anti-phospho-APBB1 (Ser347) (SL5166R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 77 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-APBB1 (Ser347)) Polyclonal Antibody, Unconjugated (SL5166R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-APBB1 (Ser347)) Polyclonal Antibody, Unconjugated (SL5166R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Cartpieces

• 
• 1

  1Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• R

  RDelete

• 
• +

  +Delete

• 
• 5

  5Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• A

  ADelete

• 
• A

  ADelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• R

  RDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout