Product Name	Metallothionein 3
Chinese Name	金属硫蛋白3抗体
Alias	Mt3; Mt-3; Mt 3; GIFB; GRIF; Metallothionein III; GIF; Growth inhibitory factor; Metallothionein3; Metallothionein-3; Metallothionein-III; MT-III; MT III; MT3_HUMAN.
Research Area	Tumour  Cell biology  immunology  Neurobiology  transcriptional regulatory factor  Zinc finger protein
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2ug/test （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	7.5kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Metallothionein 3: 1-68/68
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	MT3, Metallothionein 3, binds heavy metals. It contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. MT3 inhibits survival and neurite formation of cortical neurons in vitro. SWISS: P25713 Gene ID: 4504 Database links: Entrez Gene: 4504 Human Entrez Gene: 17751 Mouse Entrez Gene: 117038 Rat Omim: 139255 Human SwissProt: P25713 Human SwissProt: P28184 Mouse SwissProt: P37361 Rat Unigene: 73133 Human Unigene: 2064 Mouse Unigene: 11325 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Metallothionein 3) Polyclonal Antibody, Unconjugated (SL4940R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Metallothionein 3) Polyclonal Antibody, Unconjugated (SL4940R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Blank control:A549. Primary Antibody (green line): Rabbit Anti-Metallothionein 3 antibody (SL4940R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution:0.2μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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