Product Name	Osteocalcin
Chinese Name	骨钙蛋白/骨钙素抗体
Alias	Osteocalcin Propeptide; Gamma-carboxyglutamic acid-containing protein; Bone Gla-protein; BGLAP; Bone gamma carboxyglutamate protein; BGLAP; BGP; Bone gamma carboxyglutamate gla protein osteocalcin; Bone gamma carboxyglutamate protein; Bone Gla protein; Gamma carboxyglutamic acid containing protein; OC; PMF1; OSTCN_HUMAN.
literatures	Specific References  (43)     |     SL4917R has been referenced in 43 publications. [IF=13.273] Fangyu Qiao. et al. 4-Octyl itaconate modified demineralized bone matrix scaffold improves bone repair by regulating early inflammation. Chem Eng J. 2021 Dec;425:131490  WB ;  Rat.   PubMed:10.1016/j.cej.2021.131490 [IF=11.322] Jinxiu Yu. et al. Promoting osseointegration of titanium by pH-responsive releasing of H2S and optimizing polarization time for macrophages. COMPOS PART B-ENG. 2023 Mar;253:110554  IHC ;  Rat.   PubMed:10.1016/j.compositesb.2023.110554 [IF=10.652] Zhuo Liang. et al. Exosome derived from mesenchymal stem cells mediates hypoxia-specific BMP2 gene delivery and enhances bone regeneration. Chem Eng J. 2021 Oct;422:130084  IHC ;  Rabbit.   PubMed:10.1016/j.cej.2021.130084 [IF=10.319] Dan Li. et al. A HAase/NIR responsive surface on titanium implants for treating bacterial infection and improving osseointegration. J MATER SCI TECHNOL. 2022 Nov;:  IF ;  Rat.   PubMed:10.1016/j.jmst.2022.09.029 [IF=9.77]  Zheng-Zhao Liu . et al. Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3. 2020 Nov 04  IHC ;  Mouse.   PubMed:33143524 [IF=8.352] Ye Hea et al. Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation. Applied Materials Today 20 (2020) 100673  IHC ;  Rat.   PubMed:10.1016/j.apmt.2020.100673 [IF=8.31] Li, Long, et al. "Controlled dual delivery of BMP-2 and dexamethasone by nanoparticle-embedded electrospun nanofibers for the efficient repair of critical-sized rat calvarial defect." Biomaterials (2014).  IHC-P ;  Rat.   PubMed:25453952 [IF=8.025] Yixuan Li. et al. Minimally invasive bone augmentation through subperiosteal injectable hydroxylapatite/laponite/alginate nanocomposite hydrogels. INT J BIOL MACROMOL. 2023 Mar;231:123232  IHC ;  Rat.   PubMed:36681217 [IF=7.666] Maximilian Koblenzer. et al. Physiological Mineralization during In Vitro Osteogenesis in a Biomimetic Spheroid Culture Model. CELLS-BASEL. 2022 Jan;11(17):2702  IHC ;  Mouse.   PubMed:36078105 [IF=7.666] Katarzyna Klimek. et al. Could Curdlan/Whey Protein Isolate/Hydroxyapatite Biomaterials Be Considered as Promising Bone Scaffolds?—Fabrication, Characterization, and Evaluation of Cytocompatibility towards Osteoblast Cells In Vitro. CELLS-BASEL. 2022 Jan;11(20):3251  IF ;  Human.   PubMed:36291119 [IF=7.59] Zhenyin Chen. et al. Dynamic stiffening collagen-coated substrate enhances osteogenic differentiation of mesenchymal stem cells through integrin α2β1. BIOMATER SCI-UK. 2023 May;:  IF ;  Rat.   PubMed:37233200 [IF=7.31] Guangyue Li. et al. Irisin Promotes Osteogenesis by Modulating Oxidative Stress and Mitophagy through SIRT3 Signaling under Diabetic Conditions. OXID MED CELL LONGEV. 2022 Oct 10;2022:3319056  IHC ;  Rat.   PubMed:36262283 [IF=6.895] Liu M et al. Luminescent Net-like Inorganic Scaffolds with Europium Doped Hydroxyapatite for Enhanced Bone ReconstructionNanoscale.2021 Jan 6.  IF ;  Human.   PubMed:33404034 [IF=6.344] Zheng, Han. et al. miR-140-3p enhanced the osteo/odontogenic differentiation of DPSCs via inhibiting KMT5B under hypoxia condition. Int J Oral Sci. 2021 Dec;13(1):1-10  WB ;  Human.   PubMed:34876565 [IF=6.208] Samantha Ketelyn Silva. et al. Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation. INT J MOL SCI. 2022 Jan;23(12):6503  IHC ;  Rat.   PubMed:35742948 [IF=5.988] Kou Yuying. et al. Eldecalcitol prevented OVX-induced osteoporosis through inhibiting BMSCs senescence by regulating the SIRT1-Nrf2 signal. FRONT PHARMACOL. 2023 Mar;14:504  WB ;  Rat.   PubMed:36937895 [IF=5.88] Förster Y et al. The influence of different artificial extracellular matrix implant coatings on the regeneration of a critical size femur defect in rats. Mater Sci Eng C Mater Biol Appl . 2020 Nov;116:111157.  IHF-P ;  Rat.   PubMed:32806270 [IF=5.88] Qiuping Leng. et al. Demineralized bone matrix scaffold modified with mRNA derived from osteogenically pre-differentiated MSCs improves bone repair. Mat Sci Eng C-Mater. 2021 Feb;119:111601  IHC ;  Rat.   PubMed:33321645 [IF=5.854] Yi Liu. et al. Modification of COL1A1 in Autologous Adipose Tissue-Derived Progenitor Cells Rescues the Bone Phenotype in a Mouse Model of Osteogenesis Imperfecta. 2021 May 18  IF ;  Mouse.   PubMed:33950576 [IF=5.711] Zhu H et al. Direct reprogramming of mouse fibroblasts into functional osteoblasts. J Bone Miner Res. 2019 Dec 2.  ICF ;  Mouse.   PubMed:31793059 [IF=5.157] Qingnan Yan. et al. Loss of phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) in mesenchymal stem cells leads to osteopenia by impairing bone remodeling. J Biol Chem. 2022 Jan;:101639  IF ;  Mouse.   PubMed:35090892 [IF=5.051] Du Zhi. et al. Circulating exosomal circRNA_0063476 impairs expression of markers of bone growth via the miR-518c-3p/DDX6 axis in ISS. ENDOCRINOLOGY. 2022 Aug;:  IHC ;  Rat.   PubMed:35974445 [IF=4.652] Jiang et al. Andrographolide Exerts Pro-Osteogenic Effect by Activation of Wnt/β-Catenin Signaling Pathway in Vitro. (2015) Cell.Physiol.Bioche. 36:2327-39  ICC ;  Rat.   PubMed:26279437 [IF=4.545] Liu Y et al. The synergistic effect of NELL1 and adipose-derived stem cells on promoting bone formation in osteogenesis imperfecta treatment. Biomed Pharmacother. 2020 Aug;128:110235.  IHC ;  Mouse.   PubMed:32454289 [IF=4.15] Ding, Shan, et al. "A nano-micro alternating multilayer scaffold loading with rBMSCs and BMP-2 for bone tissue engineering." Colloids and Surfaces B: Biointerfaces (2015).  IHC-P ;  Rat.   PubMed:26119373 [IF=4.122] İbrahim Halilullah Erbay. et al. Bioengineering bone-on-a-chip model harnessing osteoblastic and osteoclastic resolution. ADV ENG MATER. 2022 Dec;:  IF ;  Mouse.   PubMed:10.1002/adem.202201063 [IF=3.989] Liu G et al. Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow MesenchymalStem Cells. Stem Cells Int. 2019 Apr 4;2019:7547506.  WB ;  Rat.   PubMed:31089333 [IF=3.869] Zhang Chen. et al. The Histone Demethylase KDM3B Promotes Osteo-/Odontogenic Differentiation, Cell Proliferation, and Migration Potential of Stem Cells from the Apical Papilla. Stem Cells Int. 2020;2020:8881021  WB ;  Humann.   PubMed:33082788 [IF=3.448] Xiao Y et al. Controlled delivery of recombinant human bone morphogenetic protein-2 by using glucose-sensitive core–shell nanofibers to repair the mandible defects in diabetic rats. J. Mater. Chem. B, 2019,7, 4347-4360.  ICF ;  Human.   PubMed:doi:10.1039/C9TB00613C [IF=3.328] Nan Wang. et al. Histological analysis of pulp mineralisation after severe intrusive luxation of immature molars in rats. DENT TRAUMATOL. 2023 Feb;:  IHC ;  Rat.   PubMed:36807827
Research Area	Cell biology  Signal transduction  Stem cells  Extracellular matrix
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	11kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Osteocalcin: 21-100/100
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite. Function: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Subcellular Location: Secreted. Post-translational modifications: Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. Similarity: Belongs to the osteocalcin/matrix Gla protein family. Contains 1 Gla (gamma-carboxy-glutamate) domain. SWISS: P02818 Gene ID: 632 Database links: Entrez Gene: 281646 Cow Entrez Gene: 632 Human Entrez Gene: 397530 Pig Entrez Gene: 25295 Rat Omim: 112260 Human SwissProt: P02820 Cow SwissProt: P02818 Human SwissProt: Q8HYY9 Pig SwissProt: P04640 Rat Unigene: 654541 Human Unigene: 9722 Rat 骨钙素又称骨γ-羧谷氨酸包含蛋白
Product Picture	Sample: Lane 1: Human MG63 cell lysates Lane 2: Human A549 cell lysates Primary: Anti-Osteocalcin (SL4917R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 11 kDa Observed band size: 11 kDa Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (SL4917R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (SL4917R) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin (SL0437P-Cy3) at[1:500] for 40 minutes and DAPI staining of the nu Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium clei.

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