Rabbit Anti-H2-Q10 antibody
H-2 class I histocompatibility antigen, Q10 alpha chain; HA10_MOUSE;
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Product Name H2-Q10 Chinese Name H2-Q10蛋白多克隆抗体 Alias H-2 class I histocompatibility antigen, Q10 alpha chain; HA10_MOUSE; Research Area immunology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Applications WB=1:500-2000 IHC-P=1:400-800 Flow-Cyt=1ug/Test （Paraffin sections need antigen repair）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 37kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen Recombinant mouse H2-Q10 protein Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Product Picture 
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Testis tissue lysates
Primary: Anti-H2-Q10 (SL49099R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (H2-Q10) Polyclonal Antibody, Unconjugated (SL49099R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (H2-Q10) Polyclonal Antibody, Unconjugated (SL49099R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:Raw264.7.
Primary Antibody (green line): Rabbit Anti-H2-Q10 antibody (SL49099R)
Dilution: 1ug/Test;
Secondary Antibody (green line) : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Negative control（white blue line）：PBS
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Raw264.7.
Primary Antibody (green line): Rabbit Anti- H2-Q10 antibody (SL49099R)
Dilution: 1ug/Test;
Secondary Antibody (green line) : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Negative control（white blue line）：PBS
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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