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Product Name Calponin 1 + 2 + 3 Chinese Name 钙Binding proteinCalponin抗体 Alias smooth muscle; Basic calponin; Calponin 1; Calponin 2; Calponin 3; Calponin1; Calponin2; Calponin3; Calponin-1; Calponin-2; Calponin-3; Calponin 1 basic smooth muscle; Calponin H1; Calponin H1 smooth muscle; Calponins basic; CNN 1; Cnn1; CNN2_HUMAN; Sm Calp; SMCC. Research Area Cardiovascular Cell biology Signal transduction Stem cells Binding protein Cytoskeleton Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Horse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:50-200 IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 34kDa Cellular localization cytoplasmic The cell membrane Extracellular matrix Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Calponin 1/2/3: 101-200/309 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Calponin regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an Actin- and Tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-Actin and inhibition of the Actin-activated MgATPase activity of phosphorylated myosin. Both properties are lost following phosphorylation (primarily at Serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin) and Calponin 3 (acidic Calponin) are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain.
Function:
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Tissue Specificity:
Heart and smooth muscle.
Similarity:
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain.
SWISS:
Q99439
Gene ID:
1265
Database links:Entrez Gene: 1265 Human
Omim: 602373 Human
SwissProt: Q99439 Human
Unigene: 651512 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Calponin 1 + 2 + 3) Polyclonal Antibody, Unconjugated (SL4902R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Positive control: H9C2(2% Paraformaldehyde-fixed )
Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA
Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody: rabbit Anti-Calponin 1 + 2 + 3 (SL4902R); Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA
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