Product Name	BNP
Chinese Name	脑钠素/利钠肽抗体
Alias	BNP 32; BNP(1-28); BNP(1-29); BNP(1-30); BNP(1-32); BNP(2-31); BNP(3-29); BNP(3-30); BNP(3-32); BNP(4-27); BNP(4-29); BNP(4-30); BNP(4-31); BNP(4-32); BNP(5-29); BNP(5-31); BNP(5-32); Brain natriuretic peptide 32; Brain type natriuretic peptide; Gamma brain natriuretic peptide; Natriuretic peptide B; Natriuretic peptide brain type; Natriuretic peptide precursor B; natriuretic peptide, brain type; ANFB_HUMAN; Natriuretic peptides B; natriuretic protein; NPPB; NPPB protein.
literatures	Specific References  (4)     |     SL4865R has been referenced in 4 publications. [IF=4.432] Ruomiao Li. et al. Protective effects of dioscin against isoproterenol-induced cardiac hypertrophy via adjusting PKCε/ERK-mediated oxidative stress. Eur J Pharmacol. 2021 Sep;907:174277  WB ;  Rat.   PubMed:34171391 [IF=4.069] Zhao Q et al. CTRP15 derived from cardiac myocytes attenuates TGFβ1-induced fibrotic response in cardiac fibroblasts. Cardiovasc Drugs Ther. 2020 Oct;34(5):591-604.  WB ;  Mouse.   PubMed:32424654 [IF=3.235] Cao Z et al. AIN-93 Diet as an Alternative Model to Lieber-DeCarli Diet for Alcoholic Cardiomyopathy. Alcohol Clin Exp Res. 2019 Apr 29.  IHC-P ;  Mouse.   PubMed:31034614 [IF=2.989] Lin, Yueh-Min. et al. ZAKβ Alleviates Oxidized Low-density Lipoprotein (ox-LDL)-Induced Apoptosis and B-type Natriuretic Peptide (BNP) Upregulation in Cardiomyoblast. CELL BIOCHEM BIOPHYS. 2022 Jul;:1-8  WB ;  Rat.   PubMed:35776316
Research Area	Cardiovascular  Signal transduction  Growth factors and hormones
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	3/12kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human BNP
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity. Function: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Subcellular Location: Secreted. Tissue Specificity: Brain and also in atria, but at much lower levels than ANP. Similarity: Belongs to the natriuretic peptide family. SWISS: P16860 Gene ID: 4879 Database links: Entrez Gene: 4879 Human Entrez Gene: 25105 Rat Omim: 600295 Human SwissProt: P16860 Human SwissProt: P13205 Rat
Product Picture	Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BNP Polyclonal Antibody, Unconjugated(SL4865R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BNP Polyclonal Antibody, Unconjugated(SL4865R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3, red)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

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