TEL: +86 571 56623320 EMAIL: [email protected]
Product Name Runx3 Chinese Name Runx3抗体 Alias RUNX 3; RUNX-3; Runt-related transcription factor 3; Core-binding factor, alpha 3 subunit; CBF-alpha 3; Acute myeloid leukemia 2 protein; OncogeneAML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEBP2-alpha C; PEA2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; Core-binding factor, alpha 3 subunit; CBF-alpha 3; Acute myeloid leukemia 2 protein; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEBP2-alpha C; PEA2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit. Research Area Tumour Cell biology Transporter Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Chicken, Dog, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 47kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Runx3: 31-130/415 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation (By similarity). Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain.DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1, Subcellular location in Nucleus.
Function:
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
Subunit:
Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK.
Subcellular Location:
Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm.
Post-translational modifications:
Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.
Similarity:
Contains 1 Runt domain.
SWISS:
Q13761
Gene ID:
864
Database links:Entrez Gene: 864 Human
Entrez Gene: 12399 Mouse
Omim: 600210 Human
SwissProt: Q13761 Human
SwissProt: Q64131 Mouse
Unigene: 170019 Human
Unigene: 378894 Mouse
Product Picture Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Runx3 Polyclonal Antibody, Unconjugated(SL4860R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Image provided by the Independent Validation Program, badge number 29621:A431 cells stained with Rabbit Anti-Runx3 Polyclonal Antibody at 4C overnight, followed by a Donkey Anti Rabbit FITC conjugated secondary antibody for 60 min in dark at RTU-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Runx3 Antibody(SL4860R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Scan Wechat Qrcode