Product Name	CD73
Chinese Name	CD73抗体
Alias	NT5; 5' NT; 5' nucleotidase (CD73); 5' nucleotidase precursor; 5' nucleotidase, ecto; 5' nucleotidase, ecto (CD73); 5'-NT; 5'-nucleotidase; 5'-nucleotidase; 5NTD_HUMAN; CD73; CD73 antigen; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN antibody eNT; NT antibody NT5; NT5E; NTE; Purine 5 Prime Nucleotidase.
literatures	Specific References  (26)     |     SL4834R has been referenced in 26 publications. [IF=10.761] Minji Choi. et al. Effect of Co-culture of mesenchymal stem cell and glomerulus endothelial cell to promote endothelialization under optimized perfusion flow rate in whole renal ECM scaffold. MATER TODAY BIO. 2022 Dec;17:100464  IF ;  Rat.   PubMed:36325425 [IF=9.776] Ruijing Zhao. et al. Inhalation of MSC-EVs is a noninvasive strategy for ameliorating acute lung injury. J Control Release. 2022 May;345:214  FC ;  Human.   PubMed:35307508 [IF=6.832] Wei-Cheng Tseng. et al. Hypoxic mesenchymal stem cells ameliorate acute kidney ischemia-reperfusion injury via enhancing renal tubular autophagy. Stem Cell Res Ther. 2021 Dec;12(1):1-22  FC ;  Rat.   PubMed:34183058 [IF=5.999] Jinsheng Li. et al. Micro/nano-topography Promotes Osteogenic Differentiation of Bone Marrow Stem Cells by Regulating Periostin Expression. COLLOID SURFACE B. 2022 Jul;:112700  FC ;  Rat.   PubMed:35907353 [IF=5.108] Li R et al. Metabolomic analysis reveals metabolic alterations of human peripheral blood lymphocytes by perfluorooctanoic acid. Chemosphere. 2019 Sep 7;239:124810.  ICF ;  Human.   PubMed:31520980 [IF=4.927] Yinhuan Zhang. et al. Discovery of Hepatotoxic Equivalent Markers and Mechanism of Polygonum multiflorum Thunb. by Metabolomics Coupled with Molecular Docking. MOLECULES. 2023 Jan;28(1):25  WB ;  Mouse.   PubMed:36615221 [IF=4.714] Emma Heyman. et al. Validation of multiparametric panels for bovine mesenchymal stromal cell phenotyping. CYTOM PART A. 2023 May;:  FCM ;  Bovine.   PubMed:37173856 [IF=4.648] Long et al. Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells. (2017) Aging.Di. 8:301-313  FCM ;  Rat.   PubMed:28580186 [IF=3.998] Gae Won Parket al. Topical cell-free conditioned media harvested from adipose tissue-derived stem cells promote recovery from corneal epithelial defects caused by chemical burns. Sci Rep . 2020 Jul 24;10(1):12448.  FCM ;  rat.   PubMed:32709896 [IF=3.78] Lin, Hsing-Yi, et al. "Isolation and characterization of multipotent mesenchymal stem cells adhering to adipocytes in canine bone marrow." Stem Cells and Development ja (2016).  FCM ;  Dog.   PubMed:27937753 [IF=3.427] Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900  FCM ;  Human.   PubMed:23394400 [IF=3.36] Long, Q., et al. "Bone marrow mesenchymal stem cell transplantation improves cognitive impairment via up-regulation of hippocampal GABAergic system in a rat model of chronic cerebral hypoperfusion." Neuroscience (2015).  Rat.   PubMed:26545982 [IF=2.94] Wang, Kai, et al. "Over-expression of Mash1 improves the GABAergic differentiation of bone marrow mesenchymal stem cells< i> in vitro." Brain Research Bulletin (2013).  FCM ;  Rat.   PubMed:24144723 [IF=2.88] Long, Qianfa, et al. "Genetically engineered bone marrow mesenchymal stem cells improve functional outcome in a rat model of epilepsy." Brain Research (2013).  Rat.   PubMed:23928226 [IF=2.88] Gao, Yuhua, et al. "Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells." BioMed Research International 2013 (2013).  Chicken.   PubMed:23984389 [IF=2.445] Mei X et al. Expression of VEGF, CD73 and their relationship with clinical pathology, microvessel density, and prognosis in renal cell carcinoma. Transl Androl Urol. 2020 Jun;9(3):1366-1373.  IHC-P ;  Human.   PubMed:32676421 [IF=2.03] Long, Qianfa, et al. "MRI tracking bone marrow mesenchymal stem cells labeled with ultra-small superparamagnetic iron oxide nanoparticles in a rat model of temporal lobe epilepsy." Neuroscience Letters (2015).  Rat.   PubMed:26318841 [IF=1.92] Ma, Caiyun, et al. "Cryopreservation and multipotential characteristics evaluation of a novel type of mesenchymal stem cells derived from Small Tailed Han Sheep fetal lung tissue." Cryobiology (2017).  FCM ;  Sheep.   PubMed:28284665 [IF=1.69] Gao, Yuhua, et al. "All-trans Retinoic Acid Promotes Nerve Cell Differentiation of Yolk Sac-Derived Mesenchymal Stem Cells." Applied Biochemistry and Biotechnology (2014): 1-11.  Chicken.   PubMed:25086923 [IF=1.69] Gao, Yuhua, et al. "All-trans Retinoic Acid Promotes Nerve Cell Differentiation of Yolk Sac-Derived Mesenchymal Stem Cells." Applied Biochemistry and Biotechnology (2014): 1-11.  Chicken.   PubMed:25086923 [IF=1.69] Lu, Taofeng, et al. "Isolation and Characterization of Adipose-derived Mesenchymal Stem Cells (ADSCs) from Cattle." Applied Biochemistry and Biotechnology (2014): 1-10.  Rat.   PubMed:25086927 [IF=1.461] Pei W et al. Biological characterization and pluripotent identification of ovine amniotic fluid stem cells. Cytotechnology. 2018 Jun;70(3):1009-1021.  IF&FCM ;  ovine embryo.   PubMed:29502286 [IF=1.41] Lu T et al. In vitro culture and biological properties of broiler adipose‑derived stem cells.Exp Ther Med. 2018 Sep;16(3):2399-2407.  ICF&FITC ;  broiler.   PubMed:30210592 [IF=1.28] Chen, Jia, et al. "Biological characterization of metanephric mesenchymal stem cells from the Beijing duck." Experimental and Therapeutic Medicine 11.2 (2016): 439-447.  IF(ICC) ;  Others.   PubMed:26893628 [IF=0.96] Wang, Chenghe, et al. "Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD." Official Journal of the Brazilian Society of Urology 41.4 (2015): 764-772.  Rat.   PubMed:26401871 [IF=0.918] Chen F et al. The biological characteristics of sheep umbilical cord mesenchymal stem cells.Can J Vet Res. 2018 Jul;82(3):216-224.  ICF ;  lamb.   PubMed:30026647
Research Area	Cardiovascular  Cell biology  immunology  Cell adhesion molecule  Cell Surface Molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg/Test IF=1:100-500  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	63kDa
Detection molecular weight	70kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CD73/NT5: 151-250/574
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] Function: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Subcellular Location: Cell membrane. DISEASE: Defects in NT5E are the cause of calcification of joints and arteries (CAJA). A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. Similarity: Belongs to the 5'-nucleotidase family. SWISS: P21589 Gene ID: 4907 Database links: Entrez Gene: 4907 Human Omim: 129190 Human SwissProt: P21589 Human Unigene: 153952 Human
Product Picture	Sample: Kidney (mouse)l Lysate at 40 ug Primary: Anti- CD73 (SL4834R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 65 kD Sample: HepG2 (human)Cell Lysate at 40 ug Primary: Anti- CD73 (SL4834R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kD Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug Lane 2: A549 (Human) Cell Lysate at 30 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: U251 (Human) Cell Lysate at 30 ug Primary: Anti-CD73 (SL4834R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63/71 kD Observed band size: 65 kD Blank control (blue line): Raji (blue). Primary Antibody (green line): Rabbit Anti- Phospho-c-Fos (Ser32) antibody (SL4834R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol

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