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Product Name CD83 Chinese Name CD83抗体 Alias B cell activation 45kDa cell surface glycoprotein Ig superfamily; B cell activation protein; B-cell activation protein; BL11; BL11 PEN; CD83; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); CD83 antigen; CD83 molecule; CD83_HUMAN; Cell surface glycoprotein; Cell surface protein HB15; HB15; hCD83. literatures Specific References (3) | SL4826R has been referenced in 3 publications.[IF=6.203] Huang J et al. E6-regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions. International Journal of Oncology 56, no. 4 (2020): 921-931. IHC-P ; Human.[IF=2.485] Ju et al. An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model. (2016) BMC.Immunol. 17:31 IHC ; Mouse.[IF=2.029] Jian Zheng. et al. Higher CD1a Levels Correlate with PD-L1 Expression and Predict Worse Overall Survival in Triple-Negative Breast Carcinoma. Breast Care. ;:1-9 IHC ; Human.Research Area lymphocyte t-lymphocyte b-lymphocyte Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=3ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 21kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CD83: 32-100/205 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The CD83 antigen is a 186 amino acid single chain glycoprotein. This molecule is a member of the immunoglobulin superfamily and is composed of an extracellular V type immunoglobulin-like single domain, a transmembrane region, and a short, 40 amino acid cytoplasmic tail. CD83 antigen undergoes extensive post translational glycosylation, as the determined Mr is twice the predicted size of the core protein. However, CD83+ cells have a unique cell surface immunophenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage. CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also coexpress T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36) and cytokine receptors, as well as other cell surface molecules.
Function:
May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.
Subunit:
Monomer.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Tissue Specificity:
Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells.
Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
SWISS:
Q01151
Gene ID:
9308
Database links:Entrez Gene: 9308 Human
Entrez Gene: 12522 Mouse
Omim: 604534 Human
SwissProt: Q01151 Human
SwissProt: O88324 Mouse
Unigene: 595133 Human
Unigene: 57175 Mouse
Unigene: 20096 Rat
Product Picture Blank control: A431.
Primary Antibody (green line): Rabbit Anti-CD83 antibody (SL4826R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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