Product Name	USP3
Chinese Name	去Ubiquitin化酶3抗体
Alias	Deubiquitinating enzyme 3; EC 3.1.2.15; SIH003; Ubiquitin carboxyl-terminal hydrolase 3; Ubiquitin specific peptidase 3; Ubiquitin specific processing protease 3; Ubiquitin Specific Protease 3; Ubiquitin thioesterase 3; Ubiquitin thiolesterase 3; Ubiquitin-specific-processing protease 3; UBP; UBP3_HUMAN; Usp3.
Research Area	Tumour  Cell biology  immunology  Chromatin and nuclear signals  Signal transduction  Kinases and Phosphatases  Epigenetics  Ubiquitin
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	59kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human USP3: 31-130/520
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP3 (ubiquitin specific peptidase 3), also known as UBP or SIH003, is a 520 amino acid protein that contains one UBP-type zinc finger and belongs to the peptidase C19 family. Expressed ubiquitously with highest levels present in pancreas, USP3 catalyzes the conversion of a ubiquitin C-terminal thioester to a free ubiquitin and a thiol. The gene encoding USP3 maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome. Function: Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin. Subunit: Interacts (via UBP-type domain) with H2A; the interaction is less efficient than with monoubiquitinated H2A. Subcellular Location: Nucleus. Localizes preferentially with monoubiquitinated H2A to chromatin. Tissue Specificity: Expressed in all tissues examined, with strongest expression in pancreas. Similarity: Belongs to the peptidase C19 family. USP3 subfamily. Contains 1 UBP-type zinc finger. SWISS: Q9Y6I4 Gene ID: 9960 Database links: Entrez Gene: 9960 Human Entrez Gene: 235441 Mouse Entrez Gene: 363084 Rat GenBank: NM_006537 Human GenBank: NM_144937 Mouse Omim: 604728 Human SwissProt: Q9Y6I4 Human SwissProt: Q91W36 Mouse SwissProt: A1L1I5 Rat Unigene: 458499 Human Unigene: 38976 Mouse
Product Picture	Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti- USP3 (SL4806R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD Sample: A549(Human) Cell Lysate at 30 ug U2OS(Human) Cell Lysate at 30 ug Primary: Anti- USP3 (SL4806R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD

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