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Product Name NADPH oxidase 4 Chinese Name NADPH氧化酶4抗体 Alias Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; KOX 1; KOX; Kox-1; NADPH; NADPH oxidase 4; Nox4; NOX4_HUMAN; Renal NAD(P)H-oxidase; RENOX. literatures Specific References (1) | SL4730R has been referenced in 1 publications.[IF=4.114] Jianyun Zhu. et al. Apatinib suppresses lung cancer stem-like cells by complex interplay between β-catenin signaling and mitochondrial ROS accumulation. Cell Death Discov. 2021 May;7(1):1-16 WB ; Human.Research Area Tumour Cardiovascular Cell biology immunology Signal transduction The new supersedes the old Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1ug/Test ICC=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 67kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NOX4: 201-300/578 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Nox4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases Nox4 expression, which suggests a role for Nox-4 in vascular oxidative stress response.
Function:
Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity.
Subunit:
Interacts with protein disulfide isomerase (By similarity). Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4.
Subcellular Location:
Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein (Probable). Cell junction, focal adhesion (Probable). Nucleus (Probable). Note=May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.
Tissue Specificity:
Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord.
Post-translational modifications:
Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.
Similarity:
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain.
SWISS:
Q9NPH5
Gene ID:
50507
Database links:Entrez Gene: 50507 Human
Entrez Gene: 50490 Mouse
Omim: 605261 Human
SwissProt: Q9NPH5 Human
SwissProt: Q9JHI8 Mouse
Unigene: 371036 Human
还原型辅酶烟酰胺腺嘌呤二核苷酸磷酸(NADPH)oxidase 4:还原型辅酶烟胺腺嘌呤二核苷酸(Nicotinamide adenine dinucleotide reduced, NADH)位于Mitochondrion膜内,是细胞能量代谢所必需的辅酶,主要功能是经电子传递链产生ATP。NADH在维持细胞生长、分化和能量代谢中起重要的作用。Product Picture Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-NADPH oxidase 4 (SL4730R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67 kD
Observed band size: 67 kD
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NADPH oxidase 4) polyclonal Antibody, Unconjugated (SL4730R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Blank control:293T.
Primary Antibody (green line): Rabbit Anti-NADPH oxidase 4 antibody (SL4730R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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