Product Name	phospho-TCF3 (Ser39)
Chinese Name	磷酸化转录因子3抗体
Alias	TFE2_HUMAN; Transcription factor E2-alpha; BHLHB21; E2A; ITF1; E47; p75; AGM8; VDIR; AGM8A; AGM8B; TCF-3; bHLHb21Class B basic helix-loop-helix protein 21 (bHLHb21); Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Kappa-E2-binding factor; Transcription factor 3 (TCF-3); Transcription factor ITF-1;
literatures	Specific References  (1)     |     SL4662R has been referenced in 1 publications. [IF=3.382] Das D et al. Kaempferol inhibits extra-synaptic NMDAR mediated downregulation of TRkβ in rat hippocampus during hypoxia. (2018) Neuroscience.Sep 26;392:77-91.  WB ;  Rat.   PubMed:30266684
Product Type	Phosphorylated anti
Research Area	Cell biology  Chromatin and nuclear signals  transcriptional regulatory factor  Binding protein
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Dog, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	67kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human TCF3 around the phosphorylation site of Ser39: PA(p-S)LA
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Sep 2011] Function: Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region. Subunit: Forms a heterodimer with TWIST2. Forms a heterodimer with NEUROD1; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E-box element. Isoform E12 interacts with RALGAPA1 and FIGLA. Interacts with EP300. Efficient DNA binding requires dimerization with another bHLH protein (By similarity). Homodimer. Heterodimer. Interacts with PTF1A, TGFB1I1 and UBE2I. Component of a nuclear TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3. Interacts with NEUROD2. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated following NGF stimulation (By similarity). Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. SWISS: P15923 Gene ID: 6929 Database links: Entrez Gene: 6929 Human Entrez Gene: 21423 Mouse Entrez Gene: 171046 Rat SwissProt: P15923 Human SwissProt: P15806 Mouse SwissProt: P21677 Rat
Product Picture	Sample: Embryo (Mouse) Lysate at 40 ug Brain (Rat) Lysate at 40 ug Primary: Anti-phospho-TCF3 (Ser39) (SL4662R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 67 kD Observed band size: 68 kD Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-TCF3/E2A/E47(Ser39) Polyclonal Antibody, Unconjugated(SL4662R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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