TEL: +86 571 56623320 EMAIL: [email protected]
Product Name ASB10 Chinese Name 含锚蛋白重复序列-cell factor信号抑制物盒蛋白家族10抗体 Alias Ankyrin repeat and SOCS box containing 10; Ankyrin repeat and SOCS box protein 10; ASB 10; ASB-10; ASB10; ASB10_HUMAN. Research Area Cell biology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 51kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ASB10: 401-450/467 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail ASB10 is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. SOCS boxes are carboxy terminal regions of homology found in the suppressor of cytokine signaling (SOCS) family of proteins. The box region is thought to be the point of interaction between SOCS proteins and E3 ubiquitin ligases. The SOCS box couples the suppressor of cytokine signalling proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation.
Function:
May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).
Similarity:
Contains 7 ANK repeats.
Contains 1 SOCS box domain.
SWISS:
Q8WXI3
Gene ID:
136371
Database links:Entrez Gene: 136371 Human
Omim: 615054 Human
SwissProt: Q8WXI3 Human
Unigene: 647081 Human
Product Picture Sample:
Heart (Mouse) Lysate at 40 ug
Muscle (Mouse) Lysate at 40 ug
A431(Human) Cell Lysate at 30 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- ASB10 (SL4653R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- ASB10 (SL4653R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Heart (Mouse) Lysate at 40 ug
Muscle (Mouse) Lysate at 40 ug
Primary: Anti- ASB10 (SL4653R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti- ASB10 (SL4653R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 53 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ASB10) Polyclonal Antibody, Unconjugated (SL4653R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Scan Wechat Qrcode