Product Name	BRLF1
Chinese Name	立即早期癌基因BRLF1/鼻咽癌基因抗体
Alias	RTA_EBVG; barr virus; Replication and transcription activator; Rta; Immediate-early protein Rta; HHV4tp2_gp33; BRLF1 [Human herpesvirus 4 type 2]; HHV-4;
literatures	Specific References  (3)     |     SL4542R has been referenced in 3 publications. [IF=11.205] Ruo-Wen Xiao. et al. Rare POLN mutations confer risk for familial nasopharyngeal carcinoma through weakened Epstein-Barr virus lytic replication. EBIOMEDICINE. 2022 Oct;84:104267  WB ;  Human.   PubMed:36116213 [IF=3.329] Hui D et al. CD44+CD24-/low sphere-forming cells of EBV-associated gastric carcinomas show immunosuppressive effects and induce Tregs partially through production of PGE2. Exp Cell Res. 2020 May 15;390(2):111968.  WB ;  human.   PubMed:32197932 [IF=0] Granato M et al. Quercetin Interrupts the Positive Feedback Loop Between STAT3 and IL-6, Promotes Autophagy, and Reduces ROS, Preventing EBV-Driven B Cell Immortalization. Biomolecules. 2019 Sep 12;9(9). pii: E482.  WB ;  Human.   PubMed:31547402
Research Area	Tumour  Cell biology  Bacteria and viruses
Immunogen Species	Rabbit
Clonality	Polyclonal
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	66.5kDa
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from HHV4tp2 BRLF1: 81-180/605
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Expression of the Epstein-Barr virus (EBV) immediate-early (IE) protein BRLF1 induces the lytic form of viral replication in most EBV-positive cell lines. BRLF1 is a transcriptional activator that binds directly to a GC-rich motif present in some EBV lytic gene promoters. However, BRLF1 activates transcription of the other IE protein, BZLF1, through an indirect mechanism which we previously showed to require activation of the stress mitogen-activated protein kinases. Here we demonstrate that BRLF1 activates phosphatidylinositol-3 (PI3) kinase signaling in host cells. We show that the specific PI3 kinase inhibitor, LY294002, completely abrogates the ability of a BRLF1 adenovirus vector to induce the lytic form of EBV infection, while not affecting lytic infection induced by a BZLF1 adenovirus vector. Furthermore, we demonstrate that the requirement for PI3 kinase activation in BRLF1-induced transcriptional activation is promoter dependent. BRLF1 activation of the SM early promoter (which occurs through a direct binding mechanism) does not require PI3 kinase activation, whereas activation of the IE BZLF1 and early BMRF1 promoters requires PI3 kinase activation. Thus, there are clearly two separate mechanisms by which BRLF1 induces transcriptional activation. SWISS: Q3KSS7 Gene ID: 3783727 BRLF1是EBV的立即早期基因。作为一种反式激活因子，它可以调节EBV早期／晚期基因的表达．并且还可能参与裂解期病毒基因组的复制。它的表达与EBV潜伏周期向裂解周期的转换密切相关。BRLF1的蛋因白产物Rta包含CTL识别的表位，可能在病毒裂解周期的早期成为免疫系统的作用位点。对它研究还可能为某些EBV相关Tumour的筛查和治疗提供线索。

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