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Product Name PTPLB Chinese Name 蛋白酪氨酸磷酸酶PTPLB抗体 Alias 3 hydroxyacyl CoA dehydratase 2; 3-hydroxyacyl-CoA dehydratase 2; HACD2; HACD2_HUMAN; Protein tyrosine phosphatase like (proline instead of catalytic arginine) member b; Protein tyrosine phosphatase like member B; Protein-tyrosine phosphatase-like member B; PTPLB; Very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase 2. Research Area Cell biology immunology Neurobiology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 28kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PTPLB: 181-254/254 <Cytoplasmic> Lsotype IgG Purification affinity purified by Protein A Buffer Solution Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene can catalyze the third step (dehydration) in the conversion of long chain fatty acids to very long chain fatty acids. The encoded protein localizes to the endoplasmic reticulum membrane. [provided by RefSeq, Jul 2016]
Function:
Responsible for the dehydration step in very long-chain fatty acids (VLCFAs) synthesis.
Subcellular Location:
Endoplasmic reticulum membrane.
Tissue Specificity:
Highly expressed in testis, spleen, prostate, colon and heart, followed by moderate expression in thymus, ovary, small intestine, peripheral blood leukocytes, liver, skeletal muscle and pancreas. Weakly detected in kidney, placenta, brain and lung.
Similarity:
Belongs to the very long-chain fatty acids dehydratase HACD family.
SWISS:
Q9D3B1
Gene ID:
201562
Database links:Entrez Gene: 201562 Human
Entrez Gene: 70757 Mouse
SwissProt: Q6Y1H2 Human
SwissProt: Q9D3B1 Mouse
Unigene: 705480 Human
Unigene: 27286 Mouse
Product Picture Sample:
Du145(Human) Cell Lysate at 30 ug
Primary: Anti-PTPLB (SL4429R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Sample:
LOVO(Human) Cell Lysate at 30 ug
Primary: Anti-PTPLB (SL4429R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PTPLB) Polyclonal Antibody, Unconjugated (SL4429R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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