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Product Name ZNF583 Chinese Name Zinc finger protein583抗体 Alias FLJ31030; MGC133237; Zinc finger protein 583; Zinc finger protein L3-5; ZN583_HUMAN; ZNF583. Research Area transcriptional regulatory factor Zinc finger protein Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 66kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ZNF583 Lsotype IgG Purification affinity purified by Protein A Buffer Solution Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail ZNF583 (Zinc Finger Protein 583) is a Protein Coding gene. Among its related pathways are Gene Expression. GO annotations related to this gene include nucleic acid binding. An important paralog of this gene is ZNF570.
Function:
May be involved in transcriptional regulation.
Subcellular Location:
Nucleus.
Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 12 C2H2-type zinc fingers.
Contains 1 KRAB domain.
SWISS:
Q96ND8
Gene ID:
147949
Database links:Entrez Gene: 147949 Human
Entrez Gene: 213011 Mouse
SwissProt: Q96ND8 Human
SwissProt: Q3V080 Mouse
Unigene: 146854 Human
Unigene: 726280 Human
Unigene: 333558 Mouse
Product Picture Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-ZNF583 (SL4337R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kD
Observed band size: 66 kD
Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF583) Polyclonal Antibody, Unconjugated (SL4337R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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