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Product Name TIMP-1 Chinese Name 金属蛋白酶组织抑制因子-1抗体 Alias Clgi; Collagenase inhibitor; EPA; EPO; Erythroid Potentiating Activity; Fibroblast collagenase inhibitor; FLJ90373; HC; Human Collagenase Inhibitor; Metalloproteinase inhibitor 1; Metalloproteinase inhibitor 1 precursor; OTTHUMP00000023214; TIMP 1; TIMP; TIMP metallopeptidase inhibitor 1; TIMP1 protein; Tissue Inhibitor of Metalloproteinase 1; Tissue inhibitor of metalloproteinases; Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor. Research Area Tumour Cell biology Neurobiology Apoptosis Growth factors and hormones Kinases and Phosphatases Synthesis and Degradation Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 21kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen Recombinant human TIMP-1 protein: 24-207/207 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq].
Function:
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
Subcellular Location:
Secreted.
Post-translational modifications:
The activity of TIMP1 is dependent on the presence of disulfide bonds.
Similarity:
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
SWISS:
P01033
Gene ID:
7076
Database links:Entrez Gene: 7076 Human
Entrez Gene: 21857 Mouse
Omim: 305370 Human
SwissProt: P01033 Human
SwissProt: P12032 Mouse
Unigene: 522632 Human
Unigene: 8245 Mouse
Synthesis and Degradation(Synthesis and Degradation)
TIMP1是基质金属蛋白酶的抑制酶,其功能与MMP相反,能抑制Tumour细胞的浸润和转移,主要用于各种恶性Tumour如乳腺癌等的研究。TIMP-1是一种28kDa的glycoprotein,主要分泌细胞为多种结缔组织细胞,被认为是组织中MMPs活性的主要调节者,它可以和MMPs家族成员以共价键方式形成1:1的复合物,从而发挥其抑制作用。在正常生理条件下,MMPs和TIMPs之间的平衡状态是维持Extracellular matrix正常状态所必需的,一旦它们之间的比例失调,就会造成一系列的病理状态。Product Picture Sample:
Lane 1: Human TIMP-1 Protein at 500ng
Lane 2: Human TIMP-1 Protein at 50ng
Primary: Rabbit Anti-TIMP-1 Protein Antibody at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24.4kD
Observed band size: kD
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