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Product Name 5HT3B receptor Chinese Name 5-羟色胺受体3B抗体 Alias 5 HT3 B; 5 HT3B; 5 HTR3B; 5 hydroxytryptamine 3 receptor B subunit; 5 hydroxytryptamine receptor 3B; 5 hydroxytryptamine serotonin receptor 3B; 5-HT3-B; 5-HT3B; 5-HTR3B; 5-hydroxytryptamine 3 receptor B subunit; 5-hydroxytryptamine receptor 3B; 5-hydroxytryptamine serotonin receptor 3B; 5HT3B_HUMAN; 5HTR3B; HTR3B; Serotonin gated ion channel subunit; serotonin receptor 3B. literatures Specific References (1) | SL4289R has been referenced in 1 publications.[IF=4.924] Haiyang Wang. et al. A Pilot Study of Clinical Evaluation and Formation Mechanism of Irritable Bowel Syndrome-like Symptoms in Inflammatory Bowel Disease Patients in Remission. J Neurogastroenterol. 2021 Oct 30; 27(4): 612–625 WB ; Human.Research Area Neurobiology Signal transduction Channel protein G protein-coupled receptor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Cow, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 50kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human 5HTR3B/5HT3B receptor: 101-200/441 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.
Function:
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.
Subunit:
Forms a pentaheteromeric complex with HTR3A. Not functional as a homomeric complex.
Subcellular Location:
Cell membrane; Multi-pass membrane protein. Note=Presumably retained within the endoplasmic reticulum unless complexed with HTR3A.
Tissue Specificity:
Expressed in the brain cortex, in the caudate nucleus, the hyppocampus, the thalamus and the amygdala. Detected in the kidney and testis as well as in monocytes of the spleen, small and large intestine, uterus, prostate, ovary and placenta.
Post-translational modifications:
N-glycosylation required for membrane localization.
Similarity:
Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3B sub-subfamily.
SWISS:
O95264
Gene ID:
9177
Database links:Entrez Gene: 9177 Human
Omim: 604654 Human
SwissProt: O95264 Human
Unigene: 241377 Human
Product Picture Sample: Lovo Cell (Human) Lysate at 40 ug
Primary: Anti- 5HT3B receptor (SL4289R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 55 kD
Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti- 5HT3B receptor (SL4289R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT3B receptor) Polyclonal Antibody, Unconjugated (SL4289R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control(blue):293T Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-5HT3B receptor Phospho-c-Fos (Thr325)/AF488 Conjugated antibody (SL4289R-AF488), Dilution: 1ug in 100 ul 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
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