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Product Name Nck beta Chinese Name NCK衔接蛋白2抗体 Alias Cytoplasmic protein NCK2; NCK2; GRB4; Growth factor receptor bound protein 4; NCK adaptor protein 2; Nck-2; NCK2; NCK2_HUMAN; NCKbeta; Non-catalytic region of tyrosine kinase adaptor protein 2; Noncatalytic region of tyrosine kinase beta; SH2/SH3 adaptor protein NCK-beta; SH2/SH3 adaptor protein NCK-beta. Research Area Tumour Cell biology immunology Chromatin and nuclear signals Signal transduction Cyclin Cell type markers Cytoskeleton Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Chicken, Dog, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 43kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NCK2/Nck beta: 301-380/380 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
Function:
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
Subunit:
Interacts with DOCK1, LIMS1 and TGFB1I1. Part of a complex containing PPP1R15B, PP1 and NCK2. Interacts with FASLG (By similarity). Interacts with AXL. Interacts with PAK1, PKN2 and SOS1. Interacts (via SH2 domain) with EGFR. Interacts (via SH2 domain) with DDR1.
Subcellular Location:
Cytoplasm. Endoplasmic reticulum.
Tissue Specificity:
Ubiquitous.
Post-translational modifications:
Phosphorylated.
Similarity:
Contains 1 SH2 domain.
Contains 3 SH3 domains.
SWISS:
O43639
Gene ID:
8440
Database links:Entrez Gene: 8440 Human
Entrez Gene: 17974 Mouse
Omim: 604930 Human
SwissProt: O43639 Human
SwissProt: O55033 Mouse
Unigene: 529244 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nck beta) Polyclonal Antibody, Unconjugated (SL4280R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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