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Product Name TBRG1 Chinese Name 转化生长因子β调节蛋白1抗体 Alias NIAM; Nuclear interactor of ARF and Mdm2; Transforming growth factor beta regulator 1; TBRG1; TBRG-1; TBRG 1; Transforming growth factor beta regulator 1. Research Area Tumour Cell biology immunology Chromatin and nuclear signals Cyclin Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 45kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TBRG1: 28-130/411 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Acts as a growth inhibitor. Can activate TP53/p53, causes G1 arrest and collaborates with CDKN2A to restrict proliferation, but does not require either protein to inhibit DNA synthesis. Redistributes CDKN2A into the nucleoplasm. Involved in maintaining chromosomal stability. There are two isoforms.
Function:
Acts as a growth inhibitor. Can activate p53/TP53, causes G1 arrest and collaborates with CDKN2A to restrict proliferation, but does not require either protein to inhibit DNA synthesis. Redistributes CDKN2A into the nucleoplasm. Involved in maintaining chromosomal stability.
Subunit:
Interacts with CDKN2A and MDM2.
Subcellular Location:
Nucleus.
Tissue Specificity:
Widely expressed at low levels in most tissues, with highest levels in pancreas, lung and liver. Expression is decreased in primary tumors including lung, liver, breast, pancreas and kidney carcinomas, chronic lymphocytic leukemia and diffuse large B-cell lymphoma.
Post-translational modifications:
Ubiquitinated; mediated by MDM2 and leading to its subsequent proteasomal degradation.
Similarity:
Belongs to the TBRG1 family.
Contains 1 FYR C-terminal domain.
Contains 1 FYR N-terminal domain.
SWISS:
Q3YBR2
Gene ID:
84897
Database links:Entrez Gene: 84897 Human
Entrez Gene: 21376 Mouse
Omim: 610614 Human
SwissProt: Q3YBR2 Human
SwissProt: Q3UB74 Mouse
Unigene: 436410 Human
Unigene: 28689 Mouse
Unigene: 129282 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (Human pancreatic carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBRG1) Polyclonal Antibody, Unconjugated (SL4268R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBRG1) Polyclonal Antibody, Unconjugated (SL4268R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBRG1) Polyclonal Antibody, Unconjugated (SL4268R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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