Product Name	hnRNP F
Chinese Name	异质核糖核蛋白F抗体
Alias	Heterogeneous nuclear ribonucleoprotein F; Heterogeneous nuclear ribonucleoprotein F; hnRNP F; HNRNPF; hnRP F; hnRPF; mcs94 1; MGC110997; Nucleolin like protein; Nucleolin like protein mcs94 1; OK/SW cl.23; Ribonucleoprotein F; HNRPF_HUMAN.
literatures	Specific References  (1)     |     SL4205R has been referenced in 1 publications. [IF=1.664] Zhang PF et al. MicroRNA-139 suppresses hepatocellular carcinoma cell proliferation and migration by directly targeting Topoisomerase I. ONCOLOGY LETTERS 17: 1903-1913, 2019  WB ;  Human.   PubMed:10.3892/ol.2018.9746
Research Area	Cell biology  immunology  Chromatin and nuclear signals
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Pig, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	46kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human hnRNP F: 301-415/415
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	hnRNP F is part of the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). These are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. hnRNP F binds to RNAs which have guanosine-rich sequences. Function: Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state. Subunit: Identified in the spliceosome C complex. Interacts with AGO1, AGO2, TBP and TXNL4/DIM1. Subcellular Location: Nucleus, nucleoplasm. Tissue Specificity: Expressed ubiquitously. Post-translational modifications: Sumoylated. Similarity: Contains 3 RRM (RNA recognition motif) domains. SWISS: P52597 Gene ID: 3185 Database links: Entrez Gene: 3185 Human Entrez Gene: 98758 Mouse Entrez Gene: 64200 Rat Omim: 601037 Human SwissProt: Q5E9J1 Cow SwissProt: P52597 Human SwissProt: Q9Z2X1 Mouse SwissProt: Q794E4 Rat Unigene: 712955 Human Unigene: 808 Human Unigene: 422979 Mouse Unigene: 476420 Mouse Unigene: 144596 Rat
Product Picture	Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-hnRNP F Polyclonal Antibody, Unconjugated(SL4205R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-hnRNP F Polyclonal Antibody, Unconjugated(SL4205R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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