Product Name	SOX9
Chinese Name	转录因子SOX9蛋白抗体
Alias	CMD 1; CMD1; CMPD 1; CMPD1; SOX 9; Sox9; SOX9_HUMAN; SRA 1; SRA1 antibody SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal; SRY (sex determining region Y) box 9; SRY (sex determining region Y)-box 9; SRY (sex-determining region Y)-box 9 protein; Transcription factor SOX 9; Transcription factor SOX-9; transcription factor SOX9; campomelic dysplasia autosomal sex reversal.
literatures	Specific References  (27)     |     SL4177R has been referenced in 27 publications. [IF=10.273] Tang Y et al. Structure and ingredient-based biomimetic scaffolds combining with autologous bone marrow-derived mesenchymal stem cell sheets for bone-tendon healing. Biomaterials. 2020 May;241:119837.  ICF ;  Rabbit.   PubMed:32109704 [IF=6.314] Papaioannou et al. MicroRNA-140 Provides Robustness to the Regulation of Hypertrophic Chondrocyte Differentiation by the PTHrP-HDAC4 Pathway. (2015) J.Bone.Miner.Res. 30:1044-52  WB ;  Mice.   PubMed:25529628 [IF=5.443] Huang Junchao. et al. Palovarotene Can Attenuate Heterotopic Ossification Induced by Tendon Stem Cells by Downregulating the Synergistic Effects of Smad and NF-κB Signaling Pathway following Stimulation of the Inflammatory Microenvironment. STEM CELLS INT. 2022;2022:1560943  WB,IHC ;  Rat.   PubMed:35530413 [IF=5.201] Luo Liwen. et al. Cartilage Endplate Stem Cells Transdifferentiate Into Nucleus Pulposus Cells via Autocrine Exosomes. Front Cell Dev Biol. 2021 Mar;9:482  WB ;  Rat.   PubMed:33748142 [IF=5.201] Li Taotao. et al. Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development. Front Cell Dev Biol. 2021 Mar;9:650  IF ;  Sheep.   PubMed:33816472 [IF=5.195] Dong Mao. et al. Galunisertib attenuates progression of trauma-induced heterotopic ossification via blockage of Smad2/3 signaling in mice. EUR J PHARMACOL. 2022 Aug;928:175109  IHC ;  Mouse.   PubMed:35738451 [IF=5.068] Chen XY et al. Pulsed Magnetic Field Stimuli Can Promote Chondrogenic Differentiation of Superparamagnetic Iron Oxide Nanoparticles-Labeled Mesenchymal Stem Cells in Rats.(2018) J Biomed Nanotechnol. 14(12):2135-2145.  WB ;  Rat.   PubMed:30305220 [IF=5.008] Montorsi, Lucia, et al. "Loss of zfp36 expression in colorectal cancer correlates to wnt/ß-catenin activity and enhances epithelial-to-mesenchymal transition through upregulation of zeb1, sox9 and macc1." Oncotarget (2016).  WB ;  Human.   PubMed:27463018 [IF=4.375] Sun Tianyu. et al. The Human Positive Cofactor 4 is a Promising Chemotherapeutic Target in Lung Adenocarcinoma. J Oncol. 2021;2021:9958483  IF ;  Human.   PubMed:34899911 [IF=4.169] Cheng, Baixiang. et al. Distinctive Roles of Wnt Signaling in Chondrogenic Differentiation of BMSCs under Coupling of Pressure and Platelet-Rich Fibrin. Tissue Engineering and Regenerative Medicine. 2022 Apr;:1-15  WB ;  Rabbit.   PubMed:35467329 [IF=3.923] Fang DP et al. Platelet‐rich plasma promotes the regeneration of cartilage engineered by mesenchymal stem cells and collagen hydrogel via the TGF‐β/SMAD signaling pathway. J Cell Physiol. 2019;1–11.  WB ;  Rabbit.   PubMed:doi:10.1002/jcp.28211 [IF=3.414] Lu H et al. Comparative evaluation of book‐type acellular bone scaffold and fibrocartilage scaffold for bone‐tendon healing. J Orthop Res. 2019 Apr 12.  ICF ;  Rabbit.   PubMed:30977542 [IF=3.288] Zhang N et al. Expression of Reg IV and SOX9 and their correlation in human gastric cancer. BMC Cancer. 2018 Mar 27;18(1):344.  IHC-P ;  Human.   PubMed:29587675 [IF=3.23] Webster, Mark K., et al. "Evidence of BrdU Positive Retinal Neurons after Application of an Alpha7 Nicotinic Acetylcholine." Neuroscience (2017).  IHC ;  Rat.   PubMed:28147247 [IF=3.063] Li M et al. Comparison of bone surface and trough fixation on bone–tendon healing in a rabbit patella–patellar tendon injury model. Journal of Orthopaedic Translation. 2020.  IHC ;  Rabbit.   PubMed:doi:10.1016/j.jot.2019.12.007 [IF=3.063] Zhou YC et al. Effect of book-shaped acellular tendon scaffold with bone marrow mesenchymal stem cells sheets on bone–tendon interface healing. Chem Biol Interact. 2020 May 25;323:109074.  IF ;  rabbit.   PubMed:10.1016/j.jot.2020.02.013 [IF=2.959] Zhuang M et al. Reelin regulates male mouse reproductive capacity via the sertoli cells.(2018) J. Cell. Biochem. 2018 Oct 18  WB ;  Mouse.   PubMed:30335884 [IF=2.792] Yan, Wei. et al. Neural, adipocyte and hepatic differentiation potential of primary and secondary hair follicle stem cells isolated from Arbas Cashmere goats. BMC VET RES. 2022 Dec;18(1):1-18  IF, WB ;  Goat.   PubMed:35971123 [IF=2.784] Ma C et al.Isolation and biological characteristic evaluation of a novel type of cartilage stem/progenitor cell derived from Small‑tailed Han sheep embryos.Int J Mol Med. 2018 Jul;42(1):525-533.  ICF ;  Sheep.   PubMed:29693133 [IF=2.776] Wang HC et al. Intra-articular injection of N-acetylglucosamine and hyaluronic acid combined with PLGA scaffolds for osteochondral repair in rabbits. PLoS One. 2018 Dec 31;13(12):e0209747.  IHC-P ;  Rabbit.   PubMed:30596714 [IF=2.557] Junchao Huang. et al. Palovarotene inhibits the NF-κB signaling pathway to prevent heterotopic ossification. CLIN EXP PHARMACOL P. 2022 May 31  WB ;  Mouse.   PubMed:35638441 [IF=2.466] Jinjiang Yang. et al. Platelet-rich plasma attenuates interleukin-1β-induced apoptosis and inflammation in chondrocytes through targeting hypoxia-inducible factor-2α. Tissue Cell. 2021 Sep;:101646  WB ;  rat.   PubMed:34536814 [IF=2.447] Xuejun Dai. et al. Comparison of the differentiation abilities of bone marrow‑derived mesenchymal stem cells and adipose‑derived mesenchymal stem cells toward nucleus pulposus‑like cells in three‑dimensional culture. Exp Ther Med. 2021 Sep;22(3):1-9  WB ;  Rat.   PubMed:34373704 [IF=2.09] Wang, Wei, et al. "Overexpression of heparan sulfate 6-O-sulfotransferase-2 enhances fibroblast growth factor-mediated chondrocyte growth and differentiation." International Journal of Molecular Medicine 36.3 (2015): 825-832.  WB ;  Mouse.   PubMed:26133911 [IF=2.089] Huan He. et al. BDE-209 disturbed proliferation and differentiation of spermatogonia during mitotic process through estrogen receptor α. REPROD BIOL. 2023 Jun;23:100737  WB ;  Rat.   PubMed:36821943 [IF=1.56] Mi, Jie, et al. "Study of the effect of miR‑124 and the SOX9 target gene in Hirschsprung's disease." Molecular medicine reports 9.5 (2014): 1839-1843.  WB ;  Human.   PubMed:24604230 [IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082.  WB ;  Rat.   PubMed:27665780
Research Area	Tumour  Cell biology  immunology  Developmental biology  Chromatin and nuclear signals  Neurobiology  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	56kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SOX9: 121-220/509
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq]. Function: Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Subcellular Location: Nucleus (Potential). DISEASE: Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage. Defects in SOX9 are the cause of 46,XX sex reversal type 2 (SRXX2) [MIM:278850]. SRXX2 is a condition in which male gonads develop in a genetic female (female to male sex reversal). Similarity: Contains 1 HMG box DNA-binding domain. SWISS: P48436 Gene ID: 6662 Database links: Entrez Gene: 6662 Human Entrez Gene: 20682 Mouse Entrez Gene: 140586 Rat Omim: 608160 Human SwissProt: P48436 Human SwissProt: Q04887 Mouse SwissProt: O18896 Pig Unigene: 647409 Human Unigene: 286407 Mouse     Sox9是软骨形成过程中一个十分关键的转录因子，已经有研究表明骨形成蛋白2(bone morphogenetic protein 2,BMP2)能诱导Sox9的表达。 有学者认为；Sox9蛋白很可能与椎间盘退变有着密切的关系，Sox9是Collagen protein合成过程中的一个重要的转录因子，且在软骨的发育、成熟过程中对Collagen protein有着正向调控作用，Sox9蛋白在男性性腺调节睾丸发育中也有一定的作用。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX9) Polyclonal Antibody, Unconjugated (SL4177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX9) Polyclonal Antibody, Unconjugated (SL4177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX9) Polyclonal Antibody, Unconjugated (SL4177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX9) Polyclonal Antibody, Unconjugated (SL4177R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX9) Polyclonal Antibody, Unconjugated (SL4177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control:293T. Primary Antibody (green line): Rabbit Anti-SOX9 antibody (SL4177R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control (blue line): U251 (fixed with 2% paraformaldehyde (10 min , then permeabilized) with 90% ice-cold methanol for 20 min on ice). Primary Antibody (green line): Rabbit Anti- SOX9 antibody (SL4177R),dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

Cartpieces

• 
• 1

  1Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• R

  RDelete

• 
• +

  +Delete

• 
• 1

  1Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• C

  CDelete

• 
• C

  CDelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• R

  RDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout