Product Name	Twist2
Chinese Name	TWIST蛋白2抗体抗体
Alias	bHLHa39; Class A basic helix-loop-helix protein 39; Dermis expressed protein 1; Dermis-expressed protein 1; DERMO 1; Dermo-1; DERMO1; MGC117334; Twist 2; Twist homolog 2 (Drosophila); Twist homolog 2; Twist related bHLH protein Dermo1; Twist related protein 2; Twist-related protein 2; Twist2; TWST2_HUMAN.
literatures	Specific References  (1)     |     SL4173R has been referenced in 1 publications. [IF=6.304] Yawei Wang. et al. Distinct Ring1b complexes defined by DEAD-box helicases and EMT transcription factors synergistically enhance E-cadherin silencing in breast cancer. Cell Death Dis. 2021 Feb;12(2):1-14  WB ;  Human.   PubMed:33608512
Research Area	immunology  Chromatin and nuclear signals  Signal transduction  Stem cells  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	18kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Twist2: 75-160/160
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Twist2 (Twist homolog 2) is a basic helix-loop-helix (bHLH) transcription factor which acts as a transcriptional repressor. It binds to the E-box consensus sequence 5'-CANNTG-3' and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Efficient DNA binding requires dimerization with another bHLH protein. Twist2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis. Function: Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism (By similarity). Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3/E12. Also interacts with MEF2C (By similarity). Subcellular Location: Nucleus. Cytoplasm. Note=Mainly nuclear during embryonic development. Cytoplasmic in adult tissues. Tissue Specificity: In the embryo, highly expressed in chondrogenic cells. In embryonic skin, expressed in the undifferentiated mesenchymal layer beneath the epidermis which later develops into the dermis. Expressed in early myeloid cells but not in lymphoid cells in the liver. Expression also detected in the secretory ependymal epithelium of the choroid plexus primordium. In the adult, expressed in secreting glandular tissues and tubules. DISEASE: Focal facial dermal dysplasia 3, Setleis type (FFDD3) [MIM:227260]: A form of focal facial dermal dysplasia, a group of developmental defects characterized by bitemporal or preauricular skin lesions resembling aplasia cutis congenita. FFDD3 is characterized by distinctive bitemporal scar-like depressions resembling forceps marks, and additional facial features, including a coarse and leonine appearance, absent eyelashes on both lids or multiple rows on the upper lids, absent Meibomian glands, slanted eyebrows, chin clefting, and hypo- or hyperpigmentation of the skin. Histologically, the bitemporal lesion is an ectodermal dysplasia with near absence of subcutaneous fat, suggesting insufficient migration of neural crest cells into the frontonasal process and the first branchial arch. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. SWISS: Q8WVJ9 Gene ID: 117581 Database links: Entrez Gene: 117581 Human Entrez Gene: 13345 Mouse Entrez Gene: 59327 Rat Omim: 607556 Human SwissProt: Q8WVJ9 Human SwissProt: Q9D030 Mouse SwissProt: P97831 Rat Unigene: 422585 Human Unigene: 9474 Mouse Unigene: 16279 Rat
Product Picture	Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Twist2 Polyclonal Antibody, Unconjugated(SL4173R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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