TEL: +86 571 56623320 EMAIL: [email protected]
Product Name TRIM25 Chinese Name 雌激素反应Zinc finger protein抗体 Alias EFP; Estrogen responsive finger protein; RNF 147; RNF147; TRI25; TRIM 25; Tripartite motif containing 25; Tripartite motif containing protein 25; Z147; Zinc finger protein 147; Zinc finger protein 147 (estrogen responsive finger protein); ZNF 147; ZNF147; TRI25_HUMAN. Research Area Tumour immunology Developmental biology Chromatin and nuclear signals Zinc finger protein Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 71kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TRIM25: 531-630/630 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to the cytoplasm. The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
Function:
Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
Subunit:
Interacts (via SPRY domain) with DDX58 (via CARD domain). Interacts (via coiled coil) with influenza A virus NS1 protein; this interaction specifically inhibits TRIM25 multimerization and TRIM25-mediated DDX58 CARD ubiquitination, thereby suppressing DDX58 signal transduction.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Ubiquitous.
Post-translational modifications:
Auto-ISGylated.
Similarity:
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
SWISS:
Q14258
Gene ID:
7706
Database links:Entrez Gene: 7706 Human
Entrez Gene: 217069 Mouse
Omim: 600453 Human
SwissProt: Q14258 Human
SwissProt: Q61510 Mouse
Unigene: 528952 Human
Unigene: 248445 Mouse
Unigene: 34382 Rat
Product Picture
Scan Wechat Qrcode