Product Name	CH25H
Chinese Name	胆固醇25羟化酶抗体
Alias	C25H; Cholesterol 25 hydroxylase; Cholesterol 25-hydroxylase; Cholesterol 25 monooxygenase; EC 1.14.99.38; EC=1.14.99.38; h25OH; CH25H_HUMAN.
Research Area	Tumour  Cell biology  Signal transduction  Kinases and Phosphatases  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:400-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Detection molecular weight	32
Cellular localization	cytoplasmic The cell membrane
Form	Lyophilized or Liquid
Concentration	1mg/ml
immunogen	Recombinant human CH25H protein
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	CH25H (Cholesterol 25-hydroxylase), also known as h25OH, is a 272 amino acid endoplasmic membrane protein that belongs to the sterol desaturase family. CH25H contains clusters of histidine residues essential for catalytic activity and is involved in cholesterol and lipid metabolism. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol leading to the repression of cholesterol biosynthetic enzymes. CH25H regulates lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H utilizes diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. Function: CH25H is involved in cholesterol and lipid metabolism and catalyses the formation of 25-hydroxycholesterol from cholesterol, leading to repression of cholesterol biosynthetic enzymes. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. Subcellular Location: Endoplasmic reticulum membrane Post-translational modifications: N-glycosylated. Similarity: Belongs to the sterol desaturase family. SWISS: O95992 Gene ID: 9023 Database links: Entrez Gene: 9023 Human Entrez Gene: 12642 Mouse Entrez Gene: 309527 Rat Omim: 604551 Human SwissProt: O95992 Human SwissProt: Q9Z0F5 Mouse SwissProt: Q4QQV7 Rat
Product Picture	Sample: Lane 1: Recombinant human CH25H protein, His(SL41322P) Primary: Anti-CH25H (SL41322R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: kDa Observed band size: 11 kDa Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CH25H) Polyclonal Antibody, Unconjugated (SL41322R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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