Product Name	PAP2B
Chinese Name	磷脂酸磷酸酶2b抗体
Alias	Dri42; Lipid phosphate phosphohydrolase 3; LPP3; PAP 2b; PAP2 beta; PAP2b; Vascular endothelial growth factor and type I collagen inducible protein; VCIP.
Research Area	Tumour  immunology  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Dog, Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	34kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PAP2B: 241-311/311
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Phosphatidic acid phosphatase type 2b (PPAP2b) is a member of the phosphatidic acid phosphatase (PAP) family that function in de novo synthesis of glycerolipids as well as in receptor activated signal transduction mediated by phospholipase D. PAP2b catalyzes the conversion of phosphatidic acid to diacylglycerol and hydrolyzes lysophosphatidic acid, ceramide-1-phosphate and sphingosine-1-phosphate. It is a homodimer glycoprotein localized at the cell plasma membrane. Expression promotes adhesion, spreading, and tyrosine phosphorylation of Fak, Shc, Cas and paxillin in endothelial cells. PAP2b induced cell-cell interactions regulate specific intracellular signaling pathways that involve p120-catenin, Fak, Akt, and GSK3? protein kinases. Function: Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA = PA > C-1-P > S-1-P. May be involved in cell adhesion and in cell-cell interactions. Subunit: Homodimer. This complex seems not to be involved in substrate recognition, it may confer only structural or functional stability. Subcellular Location: Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Tissue Specificity: Ubiquitously expressed. Highly expressed in heart and placenta. Post-translational modifications: N-glycosylated. Contains high-mannose oligosaccharides. Similarity: Belongs to the PA-phosphatase related phosphoesterase family. SWISS: O14495 Gene ID: 8613 Database links: Entrez Gene: 8613 Human Entrez Gene: 67916 Mouse Entrez Gene: 192270 Rat Omim: 607125 Human SwissProt: O14495 Human SwissProt: Q99JY8 Mouse SwissProt: P97544 Rat Unigene: 405156 Human Unigene: 619002 Human Unigene: 348326 Mouse Unigene: 12038 Rat
Product Picture	Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-PAP2B (SL4127R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 35 kD Sample: Embryo (Mouse) Lysate at 40 ug Primary: Anti-PAP2B (SL4127R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 35 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAP2B) Polyclonal Antibody, Unconjugated (SL4127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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