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Product Name MCP1 Chinese Name 单核细胞趋化蛋白1抗体 Alias MCP-1/CCL2; C-C motif chemokine 2; CCL 2; CCL2; CCL2_RAT; Chemokine (C C motif) ligand 2; Chemokine C C motif ligand 2; Chemokine CC Motif Ligand 2; GDCF 2; GDCF 2 HC11; GDCF-2; GDCF2; HC11; HSMCR30; HSMCR30; JE; MCAF; MCP 1; MCP-1; MGC9434; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je); Small inducible cytokine A2; Small inducible cytokine subfamily A (Cys Cys), member 2; Small inducible cytokine subfamily A Cys Cys member 2; Small-inducible cytokine A2; SMC CF; SMC-CF; SMCCF. Research Area Tumour immunology transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Rat, Applications WB=1:500-2000 IHC-P=1:400-800 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 14kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen Recombinant rat MCP-1: full length Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Processing at the N-terminus can regulate receptor and targetcell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. Genetic variations in CCL2 determine Mycobacterium tuberculosis susceptibility Belongs to the intercrine beta (chemokine CC) family. The MCP-1( the monocyte chemotactic protein1) has many pits that regulates the function toward sex to turn to record the factor, it can induce to have relation with inflammation with the immunity various genes turn to record.The immunity MCP-1 set turns the male signal to mainly locate the afterbirth syrup inside.
Function:
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Subunit:
Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans.
Subcellular Location:
Secreted.
Post-translational modifications:
Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
Similarity:
Belongs to the intercrine beta (chemokine CC) family.
SWISS:
P14844
Gene ID:
24770
Database links:Entrez Gene: 6347 Human
Entrez Gene: 20296 Mouse
Omim: 158105 Human
SwissProt: P13500 Human
SwissProt: P10148 Mouse
Unigene: 303649 Human
Unigene: 290320 Mouse
Unigene: 4772 Rat
Growth factors and hormones( Growth Factor and Hormones) 巨噬细胞趋化蛋白1(MCP1)具有多向性调节作用的核转录因子,它可诱导与免疫和炎症有关的多种基因转录.MCP-1免疫组化阳性信号主要位于胞浆内。Product Picture Sample:
Lane 1: Recombinant rat MCP-1 protein, GST & His
Primary: Anti-MCP1 (SL41226R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 46 kDa
Sample:
Lane 1: Lung (Rat) Lysate at 40 ug
Lane 2: Spleen (Rat) Lysate at 40 ug
Primary: Anti-MCP1 (SL41226R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14/11 kD
Observed band size: 11 kD
Sample:
Lane 1: Lung (Rat) Lysate at 40 ug
Lane 2: Spleen (Rat) Lysate at 40 ug
Primary: Anti-MCP1 (SL41226R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14/11 kD
Observed band size: 11 kD
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MCP1) Polyclonal Antibody, Unconjugated (SL41226R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Measured by its binding ability in a indirect ELISA. Immobilized Rat MCP-1 protein, GST & HisTag (Cat. SL41226P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Rat MCP-1 Antibody, the EC50 is 51.75 ng/mL.
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