Product Name	CTNNBIP1
Chinese Name	β链接素相互作用蛋白1抗体
Alias	Beta catenin interacting protein 1; Beta catenin interacting protein ICAT; Catenin beta interacting protein 1; CTNNBIP 1; ICAT; Inhibitor of beta catenin and Tcf 4; MGC15093; CNBP1_HUMAN.
literatures	Specific References  (1)     |     SL4095R has been referenced in 1 publications. [IF=6.52] Qi, Wei, et al. "Targeting the Wnt‐regulatory protein CTNNBIP1 by microRNA‐214 enhances the stemness and self‐renewal of cancer stem‐like cells in lung adenocarcinomas." STEM CELLS (2015).  IHC-F ;  Human.   PubMed:26299367
Research Area	Cell biology  immunology  Binding protein
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	9kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CTNNBIP1: 3-70/81
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	CTNNBIP1 (Catenin, beta interacting protein 1) binds to beta-catenin and prevents the interaction between beta-catenin and TCF transcription factor family members. It is a negative regulator of the Wnt signaling pathway. Function: Prevents the interaction between CTNNB1 and TCF family members, and acts as negative regulator of the Wnt signaling pathway. Subunit: Binds CTNNB1. Subcellular Location: Cytoplasm (By similarity). Nucleus (By similarity). Similarity: Belongs to the CTNNBIP1 family. SWISS: Q9NSA3 Gene ID: 56998 Database links: Entrez Gene: 56998 Human Entrez Gene: 67087 Mouse Entrez Gene: 503000 Rat Omim: 607758 Human SwissProt: Q9NSA3 Human SwissProt: Q9JJN6 Mouse Unigene: 463759 Human Unigene: 299735 Mouse Unigene: 162132 Rat
Product Picture	Sample: U937(Human) Cell Lysate at 30 ug Primary: Anti- CTNNBIP1 (SL4095R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 9 kD Observed band size: 9 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CTNNBIP1) Polyclonal Antibody, Unconjugated (SL4095R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CTNNBIP1) Polyclonal Antibody, Unconjugated (SL4095R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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