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Product Name phospho-CREM (Ser271 + Ser274) Chinese Name 磷酸化环磷酸腺苷反应元件调节蛋白抗体 Alias CREM (phospho S271 + S274); p-CREM (phospho S271 + S274); cAMP response element modulator; cAMP responsive element modulator; hCREM 2; hCREM2; ICER; Inducible cAMP early repressor; Inducible cAMP early repressor ICER; MGC111110; MGC17881; MGC41893. Product Type Phosphorylated anti Research Area Tumour Cell biology immunology transcriptional regulatory factor Kinases and Phosphatases Binding protein Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Chicken, Pig, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 37kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human CREM around the phosphorylation site of Ser271 + Ser274: AA(p-S)PG(p-S)LH Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail CREM is a a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is derived from a multiexonic gene that encodes both activators and antagonists of cAMP-inducible transcription by differential splicing. Splice variants with antagonistic function lack 2 glutamine-rich domains and block cAMP-induced transcription, whereas an isoform that includes these glutamine-rich domains is a transcriptional activator.
Function:
Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation.
Subunit:
Binds DNA as a dimer. Interacts with FHL5. Interacts with CDC34.
Subcellular Location:
Nucleus.
Tissue Specificity:
Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.
Post-translational modifications:
Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome.
Stimulated by phosphorylation.
Similarity:
Belongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.
SWISS:
Q03060
Gene ID:
1390
Database links:
Entrez Gene: 1390 Human
Entrez Gene: 12916 Mouse
Omim: 123812 Human
SwissProt: Q03060 Human
SwissProt: P27699 Mouse
Unigene: 200250 Human
Unigene: 10251 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CREM (Ser271 +Ser 274)) Polyclonal Antibody, Unconjugated (SL4066R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CREM (Ser271 +Ser 274)) Polyclonal Antibody, Unconjugated (SL4066R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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