Product Name	phospho-HMGCR (Ser872)
Chinese Name	磷酸化三羟基三甲基辅酶A还原酶抗体
Alias	phospho-HMGCR (S872); HMGCR (phospho-Ser872); HMGCR (phospho S872); 3 hydroxy 3 methylglutaryl CoA reductase; 3 hydroxy 3 methylglutaryl Coenzyme A reductase; 3 hydroxymethylglutaryl CoA reductase; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH); 3-hydroxy-3-methylglutaryl-coenzyme A reductase; 3H3M; HMDH_HUMAN; HMG CoA reductase; HMG CoAR; HMG-CoA reductase; Hydroxymethylglutaryl CoA reductase; LDLCQ3; MGC103269; Red.
literatures	Specific References  (7)     |     SL4063R has been referenced in 7 publications. [IF=7.171] Peng-Wei Yu. et al. EGCG Restricts PRRSV Proliferation by Disturbing Lipid Metabolism | Microbiology Spectrum. MICROBIOL SPECTR. 2022 Apr;:  WB ;  Monkey.   PubMed:35404086 [IF=4.966] Ying F et al. EP4 emerges as a novel regulator of bile acid synthesis and its activation protects against hypercholesterolemia.Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Sep;1863(9):1029-1040.  WB ;  Mouse.   PubMed:29890224 [IF=4.48] Lammi, Carmen, Chiara Zanoni, and Anna Arnoldi. "IAVPGEVA, IAVPTGVA, and LPYP, three peptides from soy glycinin, modulate cholesterol metabolism in HepG2 cells through the activation of the LDLR-SREBP2 pathway." Journal of Functional Foods 14 (2015): 469-478.  WB ;  Human.   PubMed:doi:10.1016/j.jff.2015.02.021 [IF=4.19] Lammi et al. Hypocholesterolaemic Activity of Lupin Peptides: Investigation on the Crosstalk between Human Enterocytes and Hepatocytes Using a Co-Culture System Including Caco-2 and HepG2 Cells. (2016) Nutrients. 8  WB ;  Human.   PubMed:27455315 [IF=3.97] Zanoni, Chiara, et al. "Investigations on the hypocholesterolaemic activity of LILPKHSDAD and LTFPGSAED, two peptides from lupin β-conglutin: Focus on LDLR and PCSK9 pathways." Journal of Functional Foods 32 (2017): 1-8.  WB ;  Human.   PubMed:doi:10.1016/j.jff.2017.02.009 [IF=3.689] Lu L et al. The atheroprotective roles of heart-protecting musk pills against atherosclerosis development in apolipoprotein E-deficient mice. Ann Transl Med. 2019 Dec;7(23):714.  WB ;  Mouse.   PubMed:32042730 [IF=3.1] Zanoni, Chiara, et al. "Hempseed Peptides Exert Hypocholesterolemic Effects with a Statin-Like Mechanism." Journal of Agricultural and Food Chemistry (2017).  Human.   PubMed:28931275
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  immunology  Signal transduction  transcriptional regulatory factor  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	97kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human HMGCR around the phosphorylation site of Ser872: NR(p-S)KI
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis. Function: This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis. Subunit: Homodimer. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Peroxisome membrane; Multi-pass membrane protein. Similarity: Belongs to the HMG-CoA reductase family. SWISS: P04035 Gene ID: 3156 Database links: Entrez Gene: 3156 Human Entrez Gene: 15357 Mouse Entrez Gene: 25675 Rat Omim: 142910 Human SwissProt: P04035 Human SwissProt: Q01237 Mouse SwissProt: P51639 Rat Unigene: 628096 Human Unigene: 643495 Human Unigene: 316652 Mouse Unigene: 9437 Rat
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- phospho-HMGCR (Ser872) (SL4063R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 97 kD Observed band size: 97 kD Sample: MCF-7(Human) Cell Lysate at 30 ug Primary: Anti- phospho-HMGCR (Ser872) (SL4063R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 97 kD Observed band size: 97 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-HMGCR (Ser872)) Polyclonal Antibody, Unconjugated (SL4063R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (phospho-HMGCR (Ser872)) Polyclonal Antibody, Unconjugated (SL4063R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-HMGCR (Ser872)) Polyclonal Antibody, Unconjugated (SL4063R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: A431. Primary Antibody (green line): Rabbit Anti-HMGCR antibody (SL4063R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control (Black line): A431 (Black). Primary Antibody (green line): Rabbit Anti-HMGCR antibody (SL4063R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. phosphopeptide non phosphopeptide

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