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Product Name ACADM Chinese Name 酰基辅酶A脱氢酶中链抗体 Alias mitochondrial antibody; ACAD 1; ACAD1; Acyl coenzyme A dehydrogenase; Acyl coenzyme A dehydrogenase C 4 to C 12 straight chain; MCAD; MCADH; Medium chain acyl CoA dehydrogenase; Medium chain fatty acyl CoA dehydrogenase; Medium chain specific acyl CoA dehydrogenase; Medium chain specific acyl CoA dehydrogenase mitochondrial; FLJ18227; FLJ93013; FLJ99884; ACADM_HUMAN. Research Area Tumour Cell biology immunology transcriptional regulatory factor Mitochondrion Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 47kDa Cellular localization cytoplasmic Mitochondrion Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ACADM: 151-250/421 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail ACADM protein is a medium chain specific (C4 to C12 straight chain) acyl Coenzyme A dehydrogenase. The enzyme catalyzes the initial step of the mitochondrial fatty acid beta oxidation pathway. ACADM expression is induced during periods of fasting, when reliance on fatty acids for energy is increased. Clinical phenotypes are associated with ACADM hereditary deficiency.
Function:
This enzyme is specific for acyl chain lengths of 4 to 16.
Subunit:
Homotetramer. Interacts with the heterodimeric electron transfer flavoprotein ETF.
Subcellular Location:
Mitochondrion matrix.
DISEASE:
Acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]: An inborn error of mitochondrial fatty acid beta-oxidation which causes fasting hypoglycemia, hepatic dysfunction and encephalopathy, often resulting in death in infancy. Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity:
Belongs to the acyl-CoA dehydrogenase family.
SWISS:
P11310
Gene ID:
34
Database links:
Entrez Gene: 34 Human
Entrez Gene: 11364 Mouse
Omim: 607008 Human
SwissProt: P11310 Human
SwissProt: P45952 Mouse
Unigene: 445040 Human
Unigene: 10530 Mouse
Unigene: 6302 Rat
Product Picture Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Stomach tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Rat Heart tissue lysates
Lane 5: Human K562 cell lysates
Lane 6: Human Hela cell lysates
Lane 7: Human THP-1 cell lysates
Primary: Anti-ACADM (SL4047R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACADM) Polyclonal Antibody, Unconjugated (SL4047R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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