Product Name	Triosephosphate isomerase
Chinese Name	磷酸丙糖异构酶抗体
Alias	TIM; TPI 1; TPI; TPI1; Triose phosphate isomerase 1; Triose phosphate isomerase; Triosephosphate isomerase 1; Triosephosphate isomerase; MGC88108; TPIS_HUMAN.
literatures	Specific References  (3)     |     SL4042R has been referenced in 3 publications. [IF=7.25] Yao, Chun, et al. "Role of FADD Phosphorylation in Regulating Glucose Homeostasis: from Proteomic Discovery to Physiological Validation." Molecular & Cellular Proteomics (2013).  WB ;  Mouse.   PubMed:23828893 [IF=5.682] Jie Jiang. et al. Glycolysis- and immune-related novel prognostic biomarkers of Ewing's sarcoma: glucuronic acid epimerase and triosephosphate isomerase 1. Aging-Us. 2021 Jul 15; 13(13): 17516–17535  IHC ;  Human.   PubMed:34233293 [IF=3.752] Xuejiao An. et al. miR-1285-3p targets TPI1 to regulate the glycolysis metabolism signaling pathway of Tibetan sheep Sertoli cells. PLOS ONE. 2022 Sep;17(9):e0270364  WB ;  Sheep.   PubMed:36137140
Research Area	Tumour  Cell biology  immunology  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	27kDa
Cellular localization	The nucleus cytoplasmic Extracellular matrix
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Triosephosphate isomerase: 151-249/249
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq] Subunit: Homodimer. Post-translational modifications: The initiator methionine for isoform 2 is removed. DISEASE: Triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]: Autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the triosephosphate isomerase family. SWISS: P60174 Gene ID: 7167 Database links: UniProtKB/Swiss-Prot: P60174.3 Entrez Gene: 281543 Cow Entrez Gene: 7167 Human Entrez Gene: 21991 Mouse Entrez Gene: 24849 Rat Omim: 190450 Human SwissProt: Q5E956 Cow SwissProt: P54714 Dog SwissProt: P60174 Human     磷酸丙糖异构酶（Triose-phosphate isomerase，通常简称为TPI或TIM）是一种能够催化丙糖磷酸异构体在二羟丙酮磷酸和D型甘油醛-3-磷酸之间转换。     磷酸丙糖异构酶在糖酵解中具有重要作用，对于有效的能量生成是必不可少的。磷酸丙糖异构酶被发现存在于几乎所有的生物体，包括哺乳动物、Insect、真菌、Botany和大多数细菌中。     如果人体缺乏磷酸丙糖异构酶，会导致严重的神经系统紊乱，该病症被称为磷酸丙糖异构酶缺乏症。
Product Picture	Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-Triosephosphate isomerasee (SL4042R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 30 kD Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti- Triosephosphate isomerase (SL4042R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TPIS Polyclonal Antibody, Unconjugated(SL4042R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Positive control(blue): RSC96 cells Isotype control(orange): Cell lines treated with rabbit IgG (SL0295P)instead of the primary antibody to confirm that primary antibody binding is specific. primary antibody(Green):Anti-Triosephosphate isomerase antibody(SL4042R),Concebtration: 5μg/10^6 cells Secondary only control(light blue): Both cell lines treated with Goat Anti-rabbit IgG/FITC antibody (SL0295G-FITC) to confirm no background signal produced from secondary antibody alone.

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