Product Name	NEDD8
Chinese Name	Ubiquitin样蛋白NEDD8抗体
Alias	NED8; NEDD 8; Neddylin; Neural precursor cell expressed developmentally down regulated 8; Neural precursor cell expressed developmentally down regulated gene 8; Rub1; Ubiquitin like protein Nedd 8; Ubiquitin like protein Nedd8.
Research Area	Tumour  immunology  Neurobiology  Signal transduction  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	(predicted: Human, Mouse, Rat, Pig, Cow, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	8.5kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NEDD8: 31-81/81
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Ubiquitin like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg12 and Hub1. Proteins of the second class include Parkin, RAD23 and DSK2, are designated ubiquitin domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not proteolytically processed or conjugated to other proteins. Rub1 and the corresponding human homolog NEDD8 are activated by the E1 ubiquitin activating enzyme UBA2, that forms isopeptide linkages between thio esters. NEDD8 shows 80% homology to ubiquitin. The best known targets of Rub1 modification are members of the cullin family. Cullins are subunits of an E3 ubiquitin ligase complex called the Skp1 /Cul1/Cdc53 F box (SCF). The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein. Rub1 modification may regulate SCF function or localization. Function: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Subunit: Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53. Subcellular Location: Nucleus. Note=Mainly nuclear. Tissue Specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes. Post-translational modifications: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function. Similarity: Belongs to the ubiquitin family. SWISS: Q15843 Gene ID: 4738 Database links: Entrez Gene: 35151 Fruit fly (Drosophila melanogaster) Entrez Gene: 4738 Human Entrez Gene: 18002 Mouse Entrez Gene: 25490 Rat Entrez Gene: 368667 Zebrafish Omim: 603171 Human SwissProt: Q9VJ33 Fruit fly (Drosophila melanogaster) SwissProt: Q15843 Human SwissProt: P29595 Mouse SwissProt: Q71UE8 Rat Unigene: 30848 Fruit fly (Drosophila melanogaster) Unigene: 531064 Human Unigene: 296566 Mouse Unigene: 216415 Rat

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