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Rabbit Anti-KLK11 antibody
Rabbit Anti-KLK11 antibody
Hippostasin; hK11; KLK 11; KLK-11; kallikrein 11; Kallikrein 11 isoform 1 preproprotein; Kallikrein 11 isoform 2 precursor; Kallikrein 11 precursor; kallikrein related peptidase 11; KLK 11; KLK11; Protease serine 20 trypsin like; Protease serine trypsin l
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  • NO.:SL3799R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Human,(predicted: Mouse,Rat,Dog,)
    Applications:WB ELISA IHC-P IHC-F Flow-Cyt IF
    concentration:1mg/ml
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Details

Product Name KLK11
Chinese Name 激肽释放酶11抗体
Alias Hippostasin; hK11; KLK 11; KLK-11; kallikrein 11; Kallikrein 11 isoform 1 preproprotein; Kallikrein 11 isoform 2 precursor; Kallikrein 11 precursor; kallikrein related peptidase 11; KLK 11; KLK11; Protease serine 20 trypsin like; Protease serine trypsin like; PRSS20; Serine protease 20; TLSP; Trypsin like protease; KLK11_HUMAN.  
Research Area Cell biology  immunology  Kinases and Phosphatases  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Human,  (predicted: Mouse, Rat, Dog, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 25kDa
Cellular localization cytoplasmic Secretory protein 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human KLK11: 201-282/282 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail Kallikrein 11 has two alternative splicing isoforms, known as the brain type and prostate type. Elevated serum levels of hK11 have been found in 70% of women with ovarian cancer and in 60% of men with prostate cancer. Analysis of this biomarker in serum may aid in the diagnosis and monitoring of ovarian and prostatic carcinoma.

Function:
Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues.

Subcellular Location:
Isoform 1: Secreted.
Isoform 2: Golgi apparatus.

Tissue Specificity:
Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level).

Post-translational modifications:
About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen.

Similarity:
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.

SWISS:
Q9UBX7

Gene ID:
11012

Database links:

Entrez Gene: 11012 Human

Entrez Gene: 56538 Mouse

Omim: 604434 Human

SwissProt: Q9UBX7 Human

SwissProt: Q9QYN3 Mouse

Unigene: 57771 Human



Product Picture
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-KLK11 antibody (SL3799R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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