Product Name	Perilipin A
Chinese Name	脂滴包被蛋白Perilipin-A抗体
Alias	Lipid droplet associated protein; Lipid droplet-associated protein; PERI; Perilipin; Perilipin-1; Perilipin 1; PerilipinA; Perilipin-A; PLIN; PLIN1; PLIN1_HUMAN.
literatures	Specific References  (1)     |     SL3789R has been referenced in 1 publications. [IF=4.05] Stelmanska, Ewa, Sylwia Szrok, and Julian Swierczynski. "Progesterone induced down regulation of hormone sensitive lipase ( Lipe) and up-regulation of G0/G1 switch 2 (G0s2) genes expression in inguinal adipose tissue of female rats is reflected by diminished rate of lipolysis." The Journal of Steroid Biochemistry and Molecular Biology (2014).  WB ;  Rat.   PubMed:25448749
Research Area	Cardiovascular  immunology  Synthesis and Degradation  Endocrinopathy
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	57kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Perilipin-1: 85-180/522
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Perilipins, members of the PAT protein family (named after lipid droplet proteins Perilipin, Adipophilin, and TIP47) are found exclusively at the surface of lipid droplets in adipocytes and steroidogenic cells. They have been suggested to function as regulators of lipolysis and triacylglycerol storage within adipose tissue. Four distinct isoforms ranging from perilipin A (57 kDa) to perilipin D (26 kDa) have been identified and they share an identical amino terminal sequences, and contain 2–6 consensus protein kinase A (PKA) phosphorylation sites. Perilipin C and D have been detected only in steroidogenic cells. Perilipin A is the most abundant form on the lipid droplets of adipocytes. The phosphorylation of perilipin by PKA, which is accompanied by the phosphorylation and translocation of hormone-sensitive lipase from the cytosol to the lipid droplets, promotes lipolysis. There is evidence for the presence of perilipin A in atheroma plaques suggesting that the protein may be involved in the development of therosclerosis by controlling as in adipocytes the hydrolysis of stored lipids. Function: Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Subunit: Interacts with ABHD5. Subcellular Location: Lipid droplet. Note=Lipid droplet surface-associated. Tissue Specificity: Adipocytes. Post-translational modifications: Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet. DISEASE: Defects in PLIN1 are the cause of familial partial lipodystrophy type 4 (FPLD4) [MIM:613877]. FPLD4 is a form of lipodystrophy characterized by loss of subcutaneous adipose tissue primarily affecting the lower limbs, insulin-resistant diabetes mellitus, hypertriglyceridemia, and hypertension. Similarity: Belongs to the perilipin family. SWISS: O60240 Gene ID: 5346 Database links: Entrez Gene: 520598 Cow Entrez Gene: 5346 Human Entrez Gene: 103968 Mouse Entrez Gene: 25629 Rat Omim: 170290 Human SwissProt: A4IFB3 Cow SwissProt: O60240 Human SwissProt: Q8CGN5 Mouse SwissProt: P43884 Rat Unigene: 103253 Human Unigene: 254917 Mouse Unigene: 9737 Rat     脂肪细胞中贮存的甘油三酯的水解是人和动物体内精细调节的动态平衡过程，与维持机体能量的动态平衡和代谢正常密切相关。Perilipin A是成熟脂肪细胞中脂滴膜上表达量较多的一种结构和功能蛋白，是多条脂解通路的终末靶点之一，对脂肪细胞的脂肪分解起着关键的调控作用.     perilipin可能在脂肪分解调控中起到“分子开关”的作用。蛋白激酶A（PKA）、细胞外信号调节激酶（ERK）等Signal transduction通路参与了脂肪分解。Tumour坏死因子仅（TNFα）、过氧化物酶体增殖物激活受体γ（PPAγ）激动剂、瘦素（leptin）均可以影响perilipin的表达。近年来研究，perilipin可通过蛋白酶体途径来调节其蛋白量的表达。脂肪分解调控中的关键蛋白perilipin对2型Diabetes、肥胖、动脉粥样硬化等多种代谢性疾病及Cardiovascular疾病相关联。
Product Picture	Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti-Perilipin A (SL3789R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD Sample:Parotid gland (Mouse) Lysate at 40 ug Primary: Anti-Perilipin A (SL3789R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti-Perilipin A (Bs- 3789R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Perilipin A ) Polyclonal Antibody, Unconjugated (SL3789R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

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