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Product Name ASF1A Chinese Name 细胞衰老相关蛋白ASF1A抗体 Alias Anti silencing function 1A; ASF1 anti silencing function 1 homolog A; CCG1 interacting factor A; CG9383; CGI 98; CGI98 protein; CIA; dASF1; hAsf1; hAsf1a; hCIA; Histone chaperone ASF1A; HSPC146; ASF1A_HUMAN. Research Area Tumour Cell biology immunology Chromatin and nuclear signals transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Pig, Cow, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 23kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ASF1A: 101-204/204 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail ASF1a belongs to the H3/H4 family of histone chaperone proteins. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication, repair and silencing.
Function:
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Subunit:
Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1. Interacts with CDAN1. Found in a cytosolic complex with CDAN1, ASF1B, IPO4 and histones H3.1 and H4.
Subcellular Location:
Nucleus.
Tissue Specificity:
Ubiquitously expressed.
Post-translational modifications:
Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase. TLK2-mediated phosphorylation at Ser-192 prevents proteasome-dependent degradation.
Similarity:
Belongs to the ASF1 family.
SWISS:
Q9Y294
Gene ID:
25842
Database links:Entrez Gene: 25842 Human
Entrez Gene: 66403 Mouse
Omim: 609189 Human
SwissProt: Q9Y294 Human
SwissProt: Q9CQE6 Mouse
Unigene: 292316 Human
Unigene: 272989 Mouse
ASF1a能够促进细胞衰老并且是与衰老有关的细胞周期停滞所必须的。HIRA/ASF1a介导的SAHF的形成过程直接促进细胞脱离细胞周期循环进入衰老阶段。研究人员还推测HIRA和ASF1a的改变可能使细胞无法脱离细胞周期并可能导致癌症的发生。Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ASF1A) Polyclonal Antibody, Unconjugated (SL3777R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-ASF1A antibody (SL3777R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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