TEL: +86 571 56623320    EMAIL: [email protected]

Rabbit Anti-HES5 antibody
Rabbit Anti-HES5 antibody
BHLHB38; Transcription factor HES-5; Class B basic helix-loop-helix protein 38 (bHLHb38); Hairy and enhancer of split 5; HES 5; HES5 protein; MGI:104876; HES5_HUMAN.
Total
(Vip priceV)
Regular members: $334.4
View History [Clear]

Details

Product Name HES5
Chinese Name 发状分裂相关增强子-5抗体
Alias BHLHB38; Transcription factor HES-5; Class B basic helix-loop-helix protein 38 (bHLHb38); Hairy and enhancer of split 5; HES 5; HES5 protein; MGI:104876; HES5_HUMAN.  
literatures
Specific References  (2)     |     SL3767R has been referenced in 2 publications.
[IF=6.119] Liu D et al. PNO1, which is negatively regulated by miR-340-5p, promotes lung adenocarcinoma progression through Notch signaling pathway. Oncogenesis. 2020 Jun 1;9(5):58.  WB ;  Human.  
[IF=2.96] Zhang, Haihua, et al. "Epidermal growth factor promotes proliferation of dermal papilla cells via Notch signaling pathway." Biochimie (2016).  WB ;  Other Species.  
Research Area Signal transduction  Stem cells  transcriptional regulatory factor  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Human, Mouse, Rat, 
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 18kDa
Cellular localization The nucleus 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human Hes5: 21-120/166 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer. [provided by RefSeq, Dec 2008]

Function:
Transcriptional repressor of genes that require a bHLH protein for their transcription

Subunit:
Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed in fetal heart and brain tumors.

Similarity:
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.

SWISS:
Q5TA89

Gene ID:
388585

Database links:

Entrez Gene: 388585 Human

Entrez Gene: 15208 Mouse

Entrez Gene: 79225 Rat

SwissProt: Q5TA89 Human

SwissProt: P70120 Mouse

SwissProt: Q03062 Rat



Product Picture
Sample:
Kidney (Rat) Lysate at 40 ug
Primary: Anti-alpha smooth muscle Actin (SL3767R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 20 kD
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HES5) Polyclonal Antibody, Unconjugated (SL3767R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HES5) Polyclonal Antibody, Unconjugated (SL3767R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Hes5 Polyclonal Antibody, Unconjugated(SL3767R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

References (0)

No References

Bought notes(bought amounts latest0)

No one bought this product
Total 0 records, divided into1 pages First Prev Next Last

User Comment(Total0User Comment Num)

  • No comment
Total 0 records, divided into1 pages First Prev Next Last
Username: Anonymous user
E-mail:
Rank:
Content:
Verification code: captcha

Call us

+86 571 56623320

Address

Room 1-315, Kongle Changqing Building, No. 160 Guangye Road,Gongshu District, Hangzhou City, Zhejiang Province, China

Join Us with

Leave a message
* To protect against spam, please pass the CAPTCHA test below.