Product Name	S100A4
Chinese Name	FSP-1抗体
Alias	FSP1; FSP-1; FSP 1; 18A2; 42A; calcium Placental protein; Calcium protein; Calvasculin; CAPL; Fibroblast specific protein 1; FSP1; Leukemia multidrug resistance associated protein; Malignant transformation suppression 1; Metastasin; MTS1; Mts1 protein; Murine placental homolog; OTTHUMP00000015467; OTTHUMP00000015468; P9KA; PEL98; Placental calcium binding protein; Placental calcium-binding protein; Protein Mts1; Protein S100 A4; Protein S100-A4; S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog); S100 calcium binding protein A4; S100 calcium-binding protein A4; S100a4; S10A4_HUMAN
literatures	Specific References  (9)     |     SL3759R has been referenced in 9 publications. [IF=15.881] Ke Li. et al. Multienzyme-like Reactivity Cooperatively Impairs Glutathione Peroxidase 4 and Ferroptosis Suppressor Protein 1 Pathways in Triple-Negative Breast Cancer for Sensitized Ferroptosis Therapy. Acs Nano. 2022;XXXX(XXX):XXX-XXX  WB ;  Mouse.   PubMed:35041395 [IF=7.419] Honghua Yue. et al. Colchicine: Emerging therapeutic effects on atrial fibrillation by alleviating myocardial fibrosis in a rat model. BIOMED PHARMACOTHER. 2022 Oct;154:113573  IF ;  Rat.   PubMed:35987161 [IF=6.543] Yang Lili. et al. Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion Formation. Oxid Med Cell Longev. 2022;2022:9226022  WB,IF ;  Rat,Human.   PubMed:35308169 [IF=3.947] Li Tao. et al. Epigallocatechin-3-Gallate Inhibits Atrial Fibrosis and Reduces the Occurrence and Maintenance of Atrial Fibrillation and its Possible Mechanisms. CARDIOVASC DRUG THER. 2023 Mar;:1-22  ICC ;  Rat.   PubMed:37000367 [IF=3.559] Yin X et al. Zinc oxide nanoparticles ameliorate collagen lattice contraction in human tenon fibroblasts. Arch Biochem Biophys. 2019 Jul 15;669:1-10.  WB ;  Human.   PubMed:31112708 [IF=3.375] Zhen Zhang. et al. Tanshinone IIA regulates fibroblast proliferation and migration and post-surgery arthrofibrosis through the autophagy-mediated PI3K and AMPK-mTOR signaling pathway. Am J Transl Res. 2021; 13(2): 565–584  IF ;  Rabbit.   PubMed:33594310 [IF=3.046] Tan HX et al. TGFβ1 is essential for MSCs-CAFs differentiation and promotes HCT116 cells migration and invasion via JAK/STAT3 signaling. Onco Targets Ther. 2019 Jul 5;12:5323-5334.  FCM ;  Human.   PubMed:31308702 [IF=2.985] Yun Liu. et al. Everolimus reduces postoperative arthrofibrosis in rabbits by inducing autophagy-mediated fibroblast apoptosis by PI3K/Akt/mTOR signaling pathway. Biochem Bioph Res Co. 2020 Nov;533:1  IF ;  Rabbit.   PubMed:32919704 [IF=2.049] Wang W et al. Ginkgo biloba extract may alleviate viral myocarditis by suppression of S100A4 and MMP‐3. J Med Virol. 2019 Jul 30.  IHC-P ;  Mouse.   PubMed:31359441
Research Area	Tumour  Cardiovascular  Cell biology  immunology  Signal transduction  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	12kDa
Cellular localization	The nucleus Extracellular matrix
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human S100A4: 15-101/101
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] Subunit: Homodimer. Interacts with PPFIBP1 in a calcium-dependent mode. Tissue Specificity: Ubiquitously expressed. Similarity: Belongs to the S-100 family. Contains 2 EF-hand domains. SWISS: P26447 Gene ID: 6275 Database links: Entrez Gene: 282343 Cow Entrez Gene: 403787 Dog Entrez Gene: 6275 Human Entrez Gene: 20198 Mouse Entrez Gene: 24615 Rat Omim: 114210 Human SwissProt: P35466 Cow SwissProt: Q9TV56 Dog SwissProt: P26447 Human SwissProt: P07091 Mouse SwissProt: P05942 Rat Unigene: 654444 Human Unigene: 3925 Mouse Unigene: 504 Rat
Product Picture	Sample:Hela Cell lysate at 30ug; Primary: Anti-S100A4 (SL3759R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size :12 kD Observed band size :15 kD Sample: Raji Cell lysate at 30ug; Primary: Anti-S100A4 (SL3759R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution; Predicted band size :12 kD Observed band size : 12 kD Paraformaldehyde-fixed, paraffin embedded (mouse bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100A4) Polyclonal Antibody, Unconjugated (SL3759R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100A4) Polyclonal Antibody, Unconjugated (SL3759R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human lung cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-S100A4 Polyclonal Antibody, Unconjugated(SL3759R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100A4) Polyclonal Antibody, Unconjugated (SL3759R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-S100A4 antibody (SL3759R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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