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Product Name Crkl Chinese Name 接头蛋白CrkL抗体 Alias Crk L; Crk like protein; Crkol; HGNC:2363; Oncogene CrkL; V crk avian sarcoma virus CT10 oncogene homolog like; V crk sarcoma virus CT10 oncogene homolog avian like; CRKL_HUMAN. Research Area Tumour immunology Signal transduction transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Pig, Cow, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 33kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Crkl: 201-303/303 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Crkl is a 34kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner. Crkl is a substitute of BCR ABL tyrosine kinase. In addition, Crkl has oncogenic potential.
Function:
May mediate the transduction of intracellular signals.
Subunit:
Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB and IRS4.
Similarity:
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
SWISS:
P46109
Gene ID:
1399
Database links:Entrez Gene: 1399 Human
Entrez Gene: 12929 Mouse
Omim: 602007 Human
SwissProt: P46109 Human
SwissProt: P47941 Mouse
Unigene: 5613 Human
Unigene: 21048 Mouse
Unigene: 451241 Mouse
接头蛋白CrkL是Crk家族成员之一,CRKL为具有SH2及SH3结构的连接蛋白,CrkL参与多种Signal transduction途径调节细胞运动、增殖及分化,在各种组织中广泛表达,在多种信号途径中起重要作用。Product Picture Tissue/cell:Mouse embryos; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Crkl Polyclonal Antibody, Unconjugated(SL3755R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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