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Product Name Phospho-Paxillin (Tyr31) Chinese Name 磷酸化桩蛋白Paxillin抗体 Alias Paxillin (phospho Y31); p-Paxillin (phospho Y31); PXN(phospho Y31); Paired box protein Pax 1; PAX 1; PAX1; Paxillin alpha; PXN; PXN protein; FLJ16691; FLJ23042; PAXI_HUMAN. Product Type Phosphorylated anti Research Area Tumour Cell biology immunology Apoptosis transcriptional regulatory factor Kinases and Phosphatases Cell Surface Molecule Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Chicken, Cow, Horse, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 64kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr31: TP(p-Y)SY Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]
Function:
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Subunit:
Binds in vitro to vinculin as well as to the SH3 domain of SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to PTK2/FAK1 but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to PTK2/FAK1. Interacts with GIT1, NUDT16L1/SDOS and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 (By similarity). Interacts with PTK2/FAK1 and PTK2B/PYK2. Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5 and PDCD10. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with PTK6. Interacts with SORBS1, PARVA and PARVB.
Subcellular Location:
Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex. Note=Colocalizes with integrins at the cell periphery.
Post-translational modifications:
Phosphorylated by MAPK1/ERK2 (By similarity). Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion.
Similarity:
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
SWISS:
P49023
Gene ID:
5829
Database links:Entrez Gene: 5829 Human
Entrez Gene: 19303 Mouse
Omim: 602505 Human
SwissProt: P49023 Human
SwissProt: Q8VI36 Mouse
Unigene: 446336 Human
Unigene: 18714 Mouse
Unigene: 75 Rat
Paxillin是一种分子量为68KDa的局部粘附蛋白,参与Tumour细胞的粘附与转移。主要用于各种恶性Tumour的研究。 Paxillin 蛋白是胞外基质细胞粘附(粘着斑)位点上肌动蛋白-膜附着的Cytoskeleton蛋白。 Paxillin 在整合蛋白的信号传导中起着非常重要的作用,整合蛋白介导的Cytoskeleton的再组织需要,paxillin 酪氨酸残基的磷酸化。 Paxillin 被粘着斑激酶 (FAK)在其118位的酪氨酸残基处磷酸化。已经证明,paxillin 118位和31位两个酪氨酸的磷酸化对于MM1癌细胞的迁移是重要的。Product Picture Paraformaldehyde-fixed, paraffin embedded (Mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Paxillin (Tyr31)) Polyclonal Antibody, Unconjugated (SL3744R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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