Product Name	Phospho-Cyclin B1 (Ser126)
Chinese Name	磷酸化周期素B1抗体
Alias	Cyclin B1 (phospho S126); Cyclin B1 (phospho-Ser126); p-Cyclin B1 (S126); p-Cyclin B1 (Ser126); p-Cyclin B1 (phospho S126); CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1.
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  immunology  Cyclin
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	48kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser126: TA(p-S)PS
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]. Function: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Subunit: Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Subcellular Location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome. Post-translational modifications: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site. Similarity: Belongs to the cyclin family. Cyclin AB subfamily. SWISS: P14635 Gene ID: 891 Database links: Entrez Gene: 891 Human Entrez Gene: 268697 Mouse Entrez Gene: 25203 Rat Omim: 123836 Human SwissProt: P14635 Human SwissProt: P24860 Mouse SwissProt: P30277 Rat Unigene: 23960 Human Unigene: 260114 Mouse Unigene: 380027 Mouse Unigene: 482545 Mouse Unigene: 9232 Rat 主要出现在G2期。Cyclin B是细胞周期调节必不可少的条件。 细胞周期素B1是细胞周期调节因子，它的异常表达将导致细胞周期发生紊乱，致使Tumour形成.
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Cyclin B1 (Ser126)) Polyclonal Antibody, Unconjugated (SL3733R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human esophageal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Cyclin B1 (Ser126)) Polyclonal Antibody, Unconjugated (SL3733R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Cyclin B1 (Ser126)) Polyclonal Antibody, Unconjugated (SL3733R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-Cyclin B1 (Ser126)) polyclonal Antibody, Unconjugated (SL3733R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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