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Product Name phospho-eIF4EBP1 (Ser65 + Thr70) Chinese Name 磷酸化eIF4EBinding protein抗体 Alias eIF4EBP1 (phospho S65/Th70); eIF4EBP1 (phospho Ser65/Thr70); p-eIF4EBP1 (Ser65/Thr70); Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1 (PHAS-I); 4EBP1_HUMAN. literatures Specific References (1) | SL3720R has been referenced in 1 publications.[IF=4.486] Han Zhang. et al. m6A methyltransferase METTL3 promotes retinoblastoma progression via PI3K/AKT/mTOR pathway. J Cell Mol Med. 2020 Nov;24(21):12368-12378 WB ; Human.Product Type Phosphorylated anti Research Area Tumour Cell biology immunology Signal transduction Apoptosis transcriptional regulatory factor Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 13kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from mouse 4EBP1 around the phosphorylation site of Ser65/Thr70: RN(p-S)PVTK(p-T)PP Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008].
Function:
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Subunit:
Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Interacts with RPTOR.
Post-translational modifications:
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1.
Similarity:
Belongs to the eIF4E-binding protein family.
SWISS:
Q60876
Gene ID:
13685
Database links:Entrez Gene: 1978 Human
Entrez Gene: 13685 Mouse
Omim: 602223 Human
SwissProt: Q13541 Human
SwissProt: Q60876 Mouse
Unigene: 411641 Human
Unigene: 6700 Mouse
Unigene: 11161 Rat
4E-BP1是一种分子量为10-12kDa 的酸性蛋白,与eIF4G 竞争结合。4E-BPs 对 eIF4E 的结合是可逆的,并依赖于 4E-BP 的磷酸化状态。非磷酸化的4E-BP1 能与 eiF4E 牢固结合,而磷酸化的4E-BP1则不能。
而一些蛋白激酶Akt、 TOR、 MAPK、S6激酶和Cdc2是已知的能通过对35、45、69位的苏氨酸残基或64位的丝氨酸残基进行磷酸化而使4E-BP1丧失对eIF4E结合能力的激酶。然而,并非所有的磷酸化事件都能同样地阻断4EBP1-eIF4E的相互作用。
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