Product Name	Phospho-p57 Kip2 (Thr310)
Chinese Name	磷酸化周期蛋白依赖激酶抑制因子1C抗体
Alias	p57 Kip2 (phospho T310); p57 Kip2 (phospho Thr310); p57 Kip2 (p-T310); Beckwith Wiedemann syndrome; BWCR; BWS; CDKI; CDKN 1C; CDKN1C; Cyclin dependent kinase inhibitor 1C; Cyclin dependent kinase inhibitor p57; KIP 2; KIP2; p57; p57 Kip 2; p57Kip2; WBS; IMAGE; CDN1C_HUMAN.
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  immunology  Cyclin
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	35kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human p57 Kip2 around the phosphorylation site of Thr310: EQ(p-T)PR
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]. Function: Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life. Subunit: Interacts with PCNA. Subcellular Location: Nucleus. Tissue Specificity: Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. Expressed in the eye. High levels are seen in the placenta while low levels are seen in the liver. DISEASE: Defects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650]. BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors. Defects in CDKN1C are the cause of intrauterine growth retardation, metaphyseal dysplasia, adrenal hypoplasia congenita, and genital anomalies (IMAGE) [MIM:614732]. A rare condition characterized by intrauterine growth restriction, metaphyseal dysplasia, congenital adrenal hypoplasia, and genital anomalies. Patients with this condition may present shortly after birth with severe adrenal insufficiency, which can be life-threatening if not recognized early and commenced on steroid replacement therapy. Other reported features in this condition include, hypercalciuria and/or hypercalcemia, craniosynostosis, cleft palate, and scoliosis. Note=Defects in CDKN1C are involved in tumor formation. Similarity: Belongs to the CDI family. SWISS: P49918 Gene ID: 1028 Database links: Entrez Gene: 1028 Human Entrez Gene: 12577 Mouse Entrez Gene: 246060 Rat Omim: 600856 Human SwissProt: P49918 Human SwissProt: P49919 Mouse SwissProt: E9PTV7 Rat SwissProt: Q69DC0 Rat Unigene: 106070 Human Unigene: 168789 Mouse Unigene: 162507 Rat p57 Kip-2调控周期蛋白依赖蛋白激酶、G1 期, 是Cyclin依赖性激酶(CDK)的抑制蛋白。它通过调控细胞周期进程，参与Tumour细胞的增殖、分化与凋亡。在多种Tumour中均发现p57,kip2表达异常，在某些Tumour中是一种独立的预后因素，与Tumour的发生、发展及预后有着密切关系。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-p57 Kip2 (Thr310)) Polyclonal Antibody, Unconjugated (SL3713R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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