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Product Name KLF8 Chinese Name KLF样转录因子8抗体 Alias Basic krueppel-like factor 3; BKLF3; DXS741; Kruppel like factor 8; Zinc finger protein 741; ZNF741. Research Area Tumour Cell biology immunology Signal transduction Apoptosis Cyclin transcriptional regulatory factor Cell differentiation Zinc finger protein Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 38kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human KLF8: 261-317 /359 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail KLF8 is a transcriptional repressor and activator. It also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G phase as a downstream target of focal adhesion kinase (FAK). Ubiquitously expressed.
Kruppel-like factor proteins all contain a characteristic C-terminal DNA binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers.
Function:
Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).
Subunit:
Interacts with corepressor CtBP2. Interacts with PIAS1, PIAS2, AND PIAS4; the interaction with each ligase sumoylates KLF8.
Subcellular Location:
Nucleus.
Tissue Specificity:
Ubiquitous.
Post-translational modifications:
Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G(1) phase. Has no effect on subcellular location.
Similarity:
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
SWISS:
O95600
Gene ID:
11279
Database links:Entrez Gene: 11279 Human
Omim: 300286 Human
SwissProt: O95600 Human
Unigene: 646614 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLF8) Polyclonal Antibody, Unconjugated (SL3649R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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