Product Name	PLASTIN3
Chinese Name	丝束蛋白T Plastin抗体
Alias	Plastin 3 (T isoform); PLS3; PLS 3; PLS-3; Plastin 3; Plastin3; Plastin-3; T fimbrin.
Research Area	Cell biology  immunology  Cell differentiation
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	69kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PLASTIN3: 401-500/630
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	T Plastin is a representative of an important family of actin-cross-linking and -bundling proteins that organize most of the actin networks in cells. T Plastin is associated with the actin core bundle of microvilli, stereocilia and microspikes in epithelial cells and was proposed to participate in the assembly of these structures. In mesenchymal cells, T plastin localizes to focal adhesions and the leading edge, a broad lamellar membrane extension harboring a dynamic branched actin network. Recent studies have shown that T plastin has the potential to be a Sezary cell-specific marker valuable for diagnostic and treatment of Sezary syndrome. Function: Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. Subunit: Monomer. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in a variety of organs, including muscle, brain, uterus and esophagus. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR Similarity: Contains 2 actin-binding domains. Contains 4 CH (calponin-homology) domains. Contains 2 EF-hand domains. SWISS: P13797 Gene ID: 5358 Database links: Entrez Gene: 5358 Human Entrez Gene: 102866 Mouse Entrez Gene: 81748 Rat Omim: 300131 Human SwissProt: P13797 Human SwissProt: Q99K51 Mouse SwissProt: Q63598 Rat Unigene: 496622 Human Unigene: 28777 Mouse Unigene: 32103 Rat 丝束(纤毛素)是肌动蛋白Binding protein家族的成员,存在于细胞支架的重组中. 丝束3是来源于纤毛素蛋白家族的Tlymphocyte特异肌动蛋白成束蛋白。近年来T Plastin在用于sezary cell综合征的研究方面较多。
Product Picture	Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-PLASTIN3 (SL3643R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69 kD Observed band size: 69 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLASTIN3) Polyclonal Antibody, Unconjugated (SL3643R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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