Product Name	DDX4
Chinese Name	DDX4抗体
Alias	DDX 4; DEAD (Asp Glu Ala Asp) box polypeptide 4; Dead box protein 4; Dead-box protein 4; MGC111074; MVH; Probable ATP dependent RNA helicase DDX4; VASA; VASA homolog; DDX4_HUMAN.
literatures	Specific References  (6)     |     SL3597R has been referenced in 6 publications. [IF=14.224] Wenqing Xu. et al. Oral exposure to polystyrene nanoplastics reduced male fertility and even caused male infertility by inducing testicular and sperm toxicities in mice. J HAZARD MATER. 2023 Jul;454:131470  IF ;  Mouse.   PubMed:37116333 [IF=4.996] Klein, Sabine. et al. Identification of chicken LOC420478 as Bucky ball equivalent and potential germ plasm organizer in birds. SCI REP-UK. 2022 Oct;12(1):1-15  IHC ;  Chicken.   PubMed:36207377 [IF=3.471] Nakami Wilkister Nabulindo. et al. Culture of Kenyan Goat (Capra hircus) Undifferentiated Spermatogonia in Feeder-Free Conditions. FRONT VET SCI. 2022; 9: 894075  ICC ;  Goat.   PubMed:35928111 [IF=3.195] Xia, Yuguo. et al. Gut microbiota involved in spermatogenic function of Sancai Lianmei granules in obese mice. N-S ARCH PHARMACOL. 2022 Sep;:1-15  IF ;  Mouse.   PubMed:36125532 [IF=1.786] Qin X et al. Efficient knock-in at the chicken ovalbumin locus using adenovirus as a CRISPR/Cas9 delivery system. 3 Biotech,2019, 9(12).  FCM ;  Chicken.   PubMed:doi:10.1007/s13205-019-1966-3 [IF=1.447] Tang X et al. Retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) restore the germline competence of in vitro cultured chicken blastodermal cells. In Vitro Cell Dev Biol Anim. 2019 Mar;55(3):169-176.  IF,WB&FCM ;  Chicken.   PubMed:30737631
Research Area	Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	80kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DDX4/MVH: 601-700/724
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Function: May play a role in germ cell development. May play a role in sperm motility. Subunit: Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL. Subcellular Location: Cytoplasm. Cytoplasm, perinuclear region. Tissue Specificity: Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. Similarity: Belongs to the DEAD box helicase family. DDX4/VASA subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. SWISS: Q9NQI0 Gene ID: 54514 Database links: Entrez Gene: 54514 Human Entrez Gene: 13206 Mouse Entrez Gene: 310090 Rat Omim: 605281 Human SwissProt: Q5W5U4 Cow SwissProt: Q9NQI0 Human SwissProt: Q61496 Mouse SwissProt: Q6GWX0 Pig SwissProt: Q64060 Rat Unigene: 223581 Human Unigene: 12818 Mouse Unigene: 198577 Rat
Product Picture	Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Rat Testis tissue lysates Primary: Anti-DDX4 (SL3597R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kDa Observed band size: 76 kDa Sample: Lane 1: Testis (Mouse) Tissue Lysate at 40 ug Primary: Anti- DDX4 (SL3597R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 78 kD

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