Product Name	GRIM19
Chinese Name	Interferon/维甲酸诱导凋亡相关基因抗体
Alias	CDA016; Cell death regulatory protein; Cell death regulatory protein GRIM-19; CGI-39; CGI39 protein; CI-B16.6; GRIM-19; GRIM 19; Complex I-B16.6; Gene associated with retinoic and IFN-induced mortality 19 protein; Gene associated with retinoic and interferon-induced mortality 19 protein; Gene associated with retinoic interferon induced mortality 19 protein; GRIM-19; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; NADH ubiquinone oxidoreductase B16.6 subunit; Ndufa13.
Research Area	Tumour  Cell biology  immunology  Signal transduction  Apoptosis  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Pig, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	16kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GRIM19: 51-144/144
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	A novel gene, Genes associated with Retinoid IFN induced Mortality (GRIM) GRIM19 gene was identified. Antisense expression of GRIM19 confers a strong resistance against IFN/RA induced death by reducing the intracellular levels of GRIM19 protein. Overexpression of GRIM19 enhances cell death in response to IFN/RA. GRIM19 is primarily a nuclear protein whose expression is induced by the IFN/RA combination. These data indicate that GRIM19 is a novel cell death regulatory molecule. Function: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes. Subunit: Complex I is composed of 45 different subunits. Interacts with CARD15, but not with CARD4. Interacts with STAT3, but not with STAT1, STAT2 and STAT5A. Interacts with HHV-8 IRF1, in the nucleus, with HPV-16 E6 and SV40 LT. Interacts with OLFM4. Subcellular Location: Mitochondrion inner membrane; Single-pass membrane protein; Matrix side. Nucleus. Note=May be translocated into the nucleus upon IFN/RA treatment. Tissue Specificity: Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis. DISEASE: Defects in NDUFA13 may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms. Similarity: Belongs to the complex I NDUFA13 subunit family. SWISS: Q9P0J0 Gene ID: 51079 Database links: Entrez Gene: 338084 Cow Entrez Gene: 51079 Human Entrez Gene: 67184 Mouse Entrez Gene: 314759 Rat Omim: 609435 Human SwissProt: Q95KV7 Cow SwissProt: Q9P0J0 Human SwissProt: Q9ERS2 Mouse Unigene: 534453 Human Unigene: 21162 Mouse GRIM19是GRIMs凋亡相关基因新家族成员之一,是一种由IFN-β联合RA（Interferon/维甲酸）诱导表达的16kDa蛋白,分布在The nucleus和胞浆中，GRIM-19是纯化的Mitochondrion呼吸链复合体I的组成成分,这种复合物为ATP合成所必需的,当Mitochondrion复合物I电子转移产生活性,GRIM-19突变体突破Mitochondrion跨膜电位,可增强细胞对凋亡刺激的敏感性，它的过高表达可以抑制Tumour细胞的增殖，促进细胞的凋亡。
Product Picture	Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-GRIM19 (SL3594R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 16 kD Observed band size: 17 kD Paraformaldehyde-fixed, paraffin embedded (mouse heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRIM19) Polyclonal Antibody, Unconjugated (SL3594R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRIM19) Polyclonal Antibody, Unconjugated (SL3594R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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