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Product Name NR1D1 Chinese Name The nucleus受体Rev-Erbα抗体 Alias EAR-1; EAR1; ERBA-RELATED 1; hRev; Nr1d1; NR1D1_HUMAN; Nuclear Receptor Rev-ErbA Alpha; Nuclear receptor subfamily 1 group D member 1; Orphan nuclear receptor NR1D1; Rev erbAalpha; Rev erbalpha; Rev-erbA-alpha; Rev-ErbAalpha; REV-ERBalpha; THRA1; THRAL; Thyroid hormone receptor alpha-like; Thyroid hormone receptor, alpha like; V-erbA related protein EAR-1; V-erbA-related protein 1. literatures Specific References (2) | SL3563R has been referenced in 2 publications.[IF=5.62] Zhao, Tianyun, et al. "Ketamine administered to pregnant rats in the second trimester causes long-lasting behavioral disorders in offspring." Neurobiology of Disease (2014). WB ; Rat.[IF=2.752] Lijun Dai. et al. The Distribution, Expression Patterns and Functional Analysis of NR1D1 and NR4A2 in the Reproductive Axis Tissues of the Male Tianzhu White Yak. Animals-Basel. 2021 Nov;11(11):3117 IHC ; Cow.Research Area Cell biology immunology Chromatin and nuclear signals Neurobiology Signal transduction Growth factors and hormones Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Cow, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 67kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NR1D1: 551-614/614 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail NR1D1, a NR1 Thyroid Hormone-Like Receptor, is encoded by the same genomic locus as, but transcribed from the opposite strand of, Thyroid Hormone Receptor Alpha (TR Alpha). NR1D1 is a target of Nuclear Receptor ROR Alpha and a transcription regulator that has been shown to affect myocyte differentiation, adipogenesis, and lipoprotein metabolism. Mice lacking NR1D1 show abnormal postnatal cerebellar development. NR1D1 expression has been documented in human skeletal muscle and a variety of mouse and rat tissues. ESTs have been isolated from human tissue libraries, including cancerous adrenal, blood, brain, breast, colon, duodenum, fetus, head/neck, kidney, lung, skeletal muscle, skin, synovium, uterus, normal brain, breast, colon, eye, heart, pancreas, pituitary, prostate, skeletal muscle, skin, testis and thyroid.
Function:
Functions as a constitutive transcriptional repressor. In collaboration with SP1, activates GJA1 transcription. Possible receptor for triiodothyronine.
Subunit:
Interacts with C1D and NR2E3. Interacts with SP1.
Subcellular Location:
Nucleus (Potential).
Tissue Specificity:
Expressed in all tissues and cell lines examined. Expressed at high levels in some squamous carcinoma cell lines.
Similarity:
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
SWISS:
P20393
Gene ID:
9572
Database links:Entrez Gene: 9572 Human
Entrez Gene: 217166 Mouse
Omim: 602408 Human
SwissProt: P20393 Human
SwissProt: Q3UV55 Mouse
Unigene: 592130 Human
Unigene: 724 Human
Unigene: 390397 Mouse
Unigene: 29848 Rat
Product Picture Sample:
BRL-3A (Rat) Cell Lysate at 40 ug
Primary: Anti- NR1D1 (SL3563R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67 kD
Observed band size: 67 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NR1D1) Polyclonal Antibody, Unconjugated (SL3563R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NR1D1/REV-ERB alpha Polyclonal Antibody, Unconjugated(SL3563R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NR1D1 Polyclonal Antibody, Unconjugated(SL3563R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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