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Product Name PNKP Chinese Name 多聚合苷酸激酶3磷酸化酶抗体 Alias PNK1; Bifunctional polynucleotide phosphatase/kinase; DNA 5' kinase/3' phosphatase; PNK 1; PNK; PNK1; PNKP; Polynucleotide 5' hydroxyl kinase; Polynucleotide kinase 3 prime phosphatase; Polynucleotide kinase 3' phosphatase; Polynucleotide Kinase; PNKP_HUMAN. Research Area Tumour Cell biology immunology Signal transduction Apoptosis transcriptional regulatory factor Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Cow, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 57kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PNK1/PNKP: 51-160/521 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Mammalian PNK catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. It plays an important function in DNA repair following ionizing radiation or oxidative damage. PNK has been reported to participate in the repair of DNA-double strand breaks via PARP-1-dependent nonhomologous end-joining.
Function:
Plays a key role in the repair of DNA damage,functioning as part of both the non-homologous end-joining (NHEJ)and base excision repair (BER) pathways. Through its two catalyticactivities, PNK ensures that DNA termini are compatible withextension and ligation by either removing 3'-phosphates from, or byphosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNAbackbone.
Subunit:
Monomer (By similarity).
Subcellular Location:
Nucleus.
Tissue Specificity:
Expressed in many tissues with highestexpression in spleen and testis, and lowest expression in smallintestine (PubMed:10446192). Expressed in higher amount inpancreas, heart and kidney and at lower levels in brain, lung andliver (PubMed:10446193).
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE:
Defects in PNKP are the cause of epilepticencephalopathy, early infantile, type 10 (EIEE10) [MIM:613402]. Adisease characterized by microcephaly, infantile-onset seizures,severe intellectual disability and delayed motor milestones withabsent speech or only achieving a few words. Most patients alsohave behavioral problems with hyperactivity. Microcephaly isprogressive and without neuronal migration or structuralabnormalities, consistent with primary microcephaly.
Similarity:
In the N-terminal section; belongs to the DNA 3'phosphatase family.
Contains 1 FHA domain.
SWISS:
Q96T60
Gene ID:
11284
Database links:Entrez Gene: 11284 Human
Omim: 605610 Human
SwissProt: Q96T60 Human
Unigene: 78016 Human
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